Protein Info for EX31_RS21000 in Rahnella sp. WP5

Annotation: beta-Ala-His dipeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01893: Xaa-His dipeptidase
  • amino acids 8 to 482 (475 residues), 594 bits, E=1e-182
• PF01546: Peptidase_M20
  • amino acids 72 to 480 (409 residues), 60.8 bits, E=1.8e-20
• PF07687: M20_dimer
  • amino acids 207 to 288 (82 residues), 34.9 bits, E=1.3e-12

Best Hits

Swiss-Prot: 76% identical to PEPD_ECOLI: Cytosol non-specific dipeptidase (pepD) from Escherichia coli (strain K12)

KEGG orthology group: K01270, aminoacylhistidine dipeptidase [EC: 3.4.13.3] (inferred from 100% identity to rah:Rahaq_0957)

MetaCyc: 76% identical to peptidase D (Escherichia coli K-12 substr. MG1655)
Cytosol nonspecific dipeptidase. [EC: 3.4.13.18]; 3.4.13.18 [EC: 3.4.13.18]; 3.4.13.18 [EC: 3.4.13.18]

Predicted SEED Role

"Aminoacyl-histidine dipeptidase (Peptidase D) (EC 3.4.13.3)" (EC 3.4.13.3)

MetaCyc Pathways

• γ-glutamyl cycle (5/6 steps found)
• glutathione degradation (DUG pathway) (2/2 steps found)
• muropeptide degradation (3/4 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Arginine and proline metabolism
• Glutathione metabolism
• Histidine metabolism
• Urea cycle and metabolism of amino groups
• beta-Alanine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.13.18 or 3.4.13.3

Protein Sequence (486 amino acids)

>EX31_RS21000 beta-Ala-His dipeptidase (Rahnella sp. WP5)
MSELSQLSPQPLWDIFAKICSIPHPSYHEEALAKHIVEWAQEKGIHAERDEVGNILLRKP
ATKGMENRKAVALQAHLDMVPQKNNDTVHDFTKDPIQPFIEDGWVKARGTTLGSDNGIGM
ASALAVLADDSVEHGPLEVLLTMTEETGMAGAFGLQPNWLQSEILINTDSETEGEIYMGC
AGGIDFTATLPLSREAVPAGFSTLKLTLKGLKGGHSGAEIHLGLGNASKLLARFLADNAD
TLGLRLLDFTGGTLRNAIPREAIVTLAVAADKADALKAAAQEFLATIQFELSAVEKKIAF
VVEAAESNAKALTAETQGKFLALLNATPNGVIRMSDDVKGVVETSLNVGVVTMKDDEVAI
NSLIRSLIDSGKEYVVSMLNSLSELAGAKSLAKGSYPGWKPEPNSPVMTLVRETYQTLFN
KTPNIMVIHAGLECGLFKKPYPDMDMVSIGPTITGPHSPDEQVEIASVELYWKLLTALLK
AIPERG