Protein Info for EX31_RS20980 in Rahnella sp. WP5

Annotation: glutamate 5-kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01027: glutamate 5-kinase
  • amino acids 5 to 365 (361 residues), 507.8 bits, E=8.7e-157
• PF00696: AA_kinase
  • amino acids 6 to 233 (228 residues), 154.8 bits, E=3.2e-49
• PF01472: PUA
  • amino acids 276 to 350 (75 residues), 75.8 bits, E=2.1e-25

Best Hits

Swiss-Prot: 92% identical to PROB_YERPG: Glutamate 5-kinase (proB) from Yersinia pestis bv. Antiqua (strain Angola)

KEGG orthology group: K00931, glutamate 5-kinase [EC: 2.7.2.11] (inferred from 100% identity to rah:Rahaq_0961)

MetaCyc: 88% identical to glutamate 5-kinase (Escherichia coli K-12 substr. MG1655)
Glutamate 5-kinase. [EC: 2.7.2.11]

Predicted SEED Role

"Glutamate 5-kinase (EC 2.7.2.11)" in subsystem Proline Synthesis (EC 2.7.2.11)

MetaCyc Pathways

• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-ornithine biosynthesis II (2/3 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-N^δ-acetylornithine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.2.11

Protein Sequence (367 amino acids)

>EX31_RS20980 glutamate 5-kinase (Rahnella sp. WP5)
MSGSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQQHAAGHRIVIVTSGAIAAGREHL
GYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHIGQMLLTRADMEDRERFLNARDT
MHALLDNHIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQRGLFTADPR
TNPEAELIREVMTIDDELRGVAGDSVSGLGTGGMATKLQAADVACRAGVDTIIAAGSLPG
VIGDVINGVSVGTRFHAMETPLENRKRWIFGAPPAGEITIDDGAVSAMMERGSSLLPKGI
REVKGDFSRGEVIRIRNLNGRDLAHGVSRYNSDAMRMIAGHHSQQISEILGYEYGPVAVH
RDDMIVS