Protein Info for EX31_RS20585 in Rahnella sp. WP5

Annotation: serine hydroxymethyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00464: SHMT
  • amino acids 9 to 386 (378 residues), 601.9 bits, E=4.4e-185
• PF00155: Aminotran_1_2
  • amino acids 84 to 373 (290 residues), 30.6 bits, E=2.1e-11

Best Hits

Swiss-Prot: 94% identical to GLYA_SERP5: Serine hydroxymethyltransferase (glyA) from Serratia proteamaculans (strain 568)

KEGG orthology group: K00600, glycine hydroxymethyltransferase [EC: 2.1.2.1] (inferred from 100% identity to rah:Rahaq_1028)

MetaCyc: 89% identical to serine hydroxymethyltransferase (Escherichia coli K-12 substr. MG1655)
4.1.2.-; Glycine hydroxymethyltransferase. [EC: 2.1.2.1]; RXN-6321 [EC: 2.1.2.1]; RXN0-5240 [EC: 2.1.2.1]

Predicted SEED Role

"Serine hydroxymethyltransferase (EC 2.1.2.1)" in subsystem Folate Biosynthesis or Glycine Biosynthesis or Glycine and Serine Utilization or LMPTP YwlE cluster or Photorespiration (oxidative C2 cycle) or Serine-glyoxylate cycle or Serine Biosynthesis (EC 2.1.2.1)

MetaCyc Pathways

• folate transformations III (E. coli) (9/9 steps found)
• folate transformations II (plants) (10/11 steps found)
• superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
• dTMP de novo biosynthesis (mitochondrial) (3/3 steps found)
• glycine degradation (3/3 steps found)
• folate polyglutamylation (4/5 steps found)
• glycine biosynthesis I (1/1 steps found)
• photorespiration II (7/10 steps found)
• purine nucleobases degradation II (anaerobic) (17/24 steps found)
• folate transformations I (9/13 steps found)
• photorespiration I (6/9 steps found)
• photorespiration III (6/9 steps found)
• glycine betaine degradation III (4/7 steps found)
• formaldehyde assimilation I (serine pathway) (8/13 steps found)
• glycine betaine degradation I (4/8 steps found)
• glycine betaine degradation II (mammalian) (1/4 steps found)

KEGG Metabolic Maps

• Cyanoamino acid metabolism
• Glycine, serine and threonine metabolism
• Methane metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.2.1

Protein Sequence (417 amino acids)

>EX31_RS20585 serine hydroxymethyltransferase (Rahnella sp. WP5)
MLKREMNIADYDAELWAAMEKEVVRQEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYP
GKRYYGGCEYVDIVEQLAIDRAKELFGADFANVQPHSGSQANFAVYTALLQPGDTILGMN
LGHGGHLTHGSPVNLSGKLYNVVPYGIDESGDIDYEDVKRQAELHKPKMIIGGFSAFSGI
VDWAKMREIADSVGAYFFVDMAHVAGLIAAGVYPNPVPHAHIVTTTTHKTLAGPRGGLIL
AKGGDEEFYKKLNSAVFPGGQGGPLMHVIAGKAVALKEAMEPEFKAYQQQVAKNAQAMVA
VVLERGYKVVSGGTHNHLFLMDLVDKNLTGKEADAALGRANITVNKNSVPNDPKSPFVTS
GVRIGTPAVTRRGFKEAEVRELAGWICDVLDNVNDEATIERVKQKVLDICARFPVYA