Protein Info for EX31_RS20580 in Rahnella sp. WP5

Annotation: 3-phenylpropionate MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 386 transmembrane" amino acids 9 to 29 (21 residues), see Phobius details amino acids 41 to 60 (20 residues), see Phobius details amino acids 68 to 89 (22 residues), see Phobius details amino acids 95 to 114 (20 residues), see Phobius details amino acids 133 to 152 (20 residues), see Phobius details amino acids 158 to 176 (19 residues), see Phobius details amino acids 205 to 228 (24 residues), see Phobius details amino acids 240 to 258 (19 residues), see Phobius details amino acids 270 to 289 (20 residues), see Phobius details amino acids 295 to 317 (23 residues), see Phobius details amino acids 329 to 351 (23 residues), see Phobius details amino acids 357 to 376 (20 residues), see Phobius details PF12832: MFS_1_like" amino acids 8 to 360 (353 residues), 189.3 bits, see alignment E=2.3e-59 PF07690: MFS_1" amino acids 9 to 305 (297 residues), 44.5 bits, see alignment E=2.1e-15 PF01306: LacY_symp" amino acids 9 to 365 (357 residues), 33.6 bits, see alignment E=4.1e-12 PF03825: Nuc_H_symport" amino acids 11 to 354 (344 residues), 40 bits, see alignment E=5.4e-14

Best Hits

Swiss-Prot: 68% identical to HCAT_ECOLI: Probable 3-phenylpropionic acid transporter (hcaT) from Escherichia coli (strain K12)

KEGG orthology group: K05820, MFS transporter, PPP family, 3-phenylpropionic acid transporter (inferred from 100% identity to rah:Rahaq_1029)

Predicted SEED Role

"Probable 3-phenylpropionic acid transporter" in subsystem Cinnamic Acid Degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (386 amino acids)

>EX31_RS20580 3-phenylpropionate MFS transporter (Rahnella sp. WP5)
MELRSTRWLALSYFTYFFSYGIFLPFWGLWLKGEGISSESIGLLLGAGLVARFLGTLIIS
PSIKDPSYLITGLRILALLTLAFAVGFWFGNGWAWLMINIAGFSLFFGPLVPLTDALAGT
LQRQINMDYGKVRVWGSIAFVIGSAVTGKLVEAYGHKAILYTLTAGVAAMLLGMLLRPSV
KPVGEPRTSGAPGISWKALLGEKPVWRFLLCVTLLQGAHAAYYGFSAIYWQDVGYSSTTI
GYLWSLGVVAEVLVFTFSKQLFSRWSARDLLLLSAVCGVVRWGMMGAFTALPWLIVMQIL
HCGTFTVCHLAGMRFIAARPRDQVIRLQSVYSALAMGGGVAVMTMVSGFLFEHLHAGLFW
VMALLAVPAIFLRPVVTPSAPVTSAR