Protein Info for EX31_RS20545 in Rahnella sp. WP5

Annotation: IscS subfamily cysteine desulfurase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02006: cysteine desulfurase IscS
  • amino acids 1 to 387 (387 residues), 684.3 bits, E=2.7e-210
• PF00266: Aminotran_5
  • amino acids 5 to 368 (364 residues), 289.6 bits, E=7.3e-90
• PF00155: Aminotran_1_2
  • amino acids 52 to 292 (241 residues), 22.8 bits, E=1e-08
• PF01212: Beta_elim_lyase
  • amino acids 68 to 208 (141 residues), 29.1 bits, E=1.2e-10
• PF01041: DegT_DnrJ_EryC1
  • amino acids 76 to 191 (116 residues), 20.7 bits, E=4.4e-08

Best Hits

Swiss-Prot: 81% identical to ISCS_PECAS: Cysteine desulfurase IscS (iscS) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K04487, cysteine desulfurase [EC: 2.8.1.7] (inferred from 98% identity to rah:Rahaq_1036)

MetaCyc: 81% identical to cysteine desulfurase IscS (Escherichia coli K-12 substr. MG1655)
Cysteine desulfurase. [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]

Predicted SEED Role

"Cysteine desulfurase (EC 2.8.1.7), IscS subfamily" in subsystem Alanine biosynthesis or Thiamin biosynthesis (EC 2.8.1.7)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (10/10 steps found)
• thiazole component of thiamine diphosphate biosynthesis I (6/6 steps found)
• superpathway of L-alanine biosynthesis (4/4 steps found)
• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• molybdopterin biosynthesis (5/6 steps found)
• L-alanine biosynthesis III (1/1 steps found)
• L-cysteine degradation IV (1/1 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (5/7 steps found)
• cytidylyl molybdenum cofactor sulfurylation (1/2 steps found)
• tRNA-uridine 2-thiolation and selenation (bacteria) (7/11 steps found)
• bis(guanylyl molybdopterin) cofactor sulfurylation (1/3 steps found)
• tRNA-uridine 2-thiolation (mammalian mitochondria) (1/4 steps found)
• tRNA-uridine 2-thiolation (yeast mitochondria) (1/4 steps found)
• tRNA-uridine 2-thiolation (thermophilic bacteria) (1/5 steps found)
• tRNA-uridine 2-thiolation (cytoplasmic) (1/8 steps found)
• [2Fe-2S] iron-sulfur cluster biosynthesis (2/10 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.7

Use Curated BLAST to search for 2.8.1.7

Protein Sequence (389 amino acids)

>EX31_RS20545 IscS subfamily cysteine desulfurase (Rahnella sp. WP5)
MKLPIYLDYSATTPVDPRVAQKMMQCLTLDGTFGNPASRSHRFGWQAEEAVDVARNQIAG
LINVDPREIVFTSGATESDNLAIKGAAHAHSAKGKHIITSQTEHKAVLDTCAQLETEGFD
VTYLAPQADGFIPPAAIEAALRPDTILVSIMQVNNEIGVVQDIATIGELCRSRGILFHVD
ATQSVGKIAIDLSELQVDLMSFSAHKIYGPKGIGALYVSRKPKVRIDAQIHGGGHERGMR
SGTLPVHQIVGMGEAYRIAKEEMTTEVARLTELRDRLWKGIQALDQVFLNGTPKHAAPNI
LNVSFASVEGESLIMALKDLAVSSGSACTSASLEPSYVLRALGLSEELAHSSLRFSLGRW
TTEEEIDYAIALVVKSVRRLRELPPVYTK