Protein Info for EX31_RS20510 in Rahnella sp. WP5

Annotation: flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00612: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
  • amino acids 9 to 352 (344 residues), 403.7 bits, E=3.8e-125
• PF04551: GcpE
  • amino acids 13 to 354 (342 residues), 471.3 bits, E=8.7e-146

Best Hits

Swiss-Prot: 93% identical to ISPG_SERP5: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG) from Serratia proteamaculans (strain 568)

KEGG orthology group: K03526, (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase [EC: 1.17.7.1] (inferred from 100% identity to rah:Rahaq_1043)

MetaCyc: 90% identical to (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin) (Escherichia coli K-12 substr. MG1655)
RXN-15878 [EC: 1.17.7.3]

Predicted SEED Role

"1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (EC 1.17.7.1)" in subsystem Isoprenoid Biosynthesis (EC 1.17.7.1)

MetaCyc Pathways

• superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) (12/12 steps found)
• taxadiene biosynthesis (engineered) (12/13 steps found)
• methylerythritol phosphate pathway I (9/9 steps found)
• methylerythritol phosphate pathway II (9/9 steps found)
• isoprene biosynthesis I (9/10 steps found)
• superpathway of ergosterol biosynthesis II (11/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Terpenoid biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.7.1 or 1.17.7.3

Protein Sequence (373 amino acids)

>EX31_RS20510 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (Rahnella sp. WP5)
MHNQAPITRRKSTRIYVGKVPVGDGAPIAVQSMTNTRTTDVAATVAQIKSLERVGVDIVR
VSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALQVAEYGVDCLRINPGNIGNESRIRS
VVDCARDKNIPIRIGVNGGSLEKDIQEKYGEPTPQALLESAMRHVDILDRLNFDQFKVSV
KASDVFLAVESYRLLAKQIVQPLHLGITEAGGLRAGSVKSAIGLGLLLSEGIGDTLRISL
AADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDV
SIIGCVVNGPGEALVSTMGVTGGHNKSGFYEDGVRQRERFDNNDMIDQLEAKIRAKAAIM
DESKRIVINHLEK