Protein Info for EX31_RS20490 in Rahnella sp. WP5

Annotation: ribosome biogenesis GTPase Der

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03594: ribosome-associated GTPase EngA
  • amino acids 3 to 462 (460 residues), 588.5 bits, E=1.3e-180
• TIGR00231: small GTP-binding protein domain
  • amino acids 4 to 157 (154 residues), 71.8 bits, E=8.4e-24
  • amino acids 207 to 372 (166 residues), 75.1 bits, E=8.2e-25
• PF02421: FeoB_N
  • amino acids 5 to 155 (151 residues), 57.8 bits, E=5.5e-19
  • amino acids 208 to 370 (163 residues), 46.2 bits, E=1.9e-15
• PF01926: MMR_HSR1
  • amino acids 5 to 119 (115 residues), 103.4 bits, E=4.3e-33
  • amino acids 209 to 326 (118 residues), 93.1 bits, E=7e-30
• PF00009: GTP_EFTU
  • amino acids 240 to 374 (135 residues), 37.1 bits, E=1.4e-12
• PF14714: KH_dom-like
  • amino acids 382 to 462 (81 residues), 93.8 bits, E=3.5e-30

Best Hits

Swiss-Prot: 88% identical to DER_YERE8: GTPase Der (der) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K03977, GTP-binding protein (inferred from 100% identity to rah:Rahaq_1047)

MetaCyc: 82% identical to 50S ribosomal subunit stability factor (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein EngA" in subsystem Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (494 amino acids)

>EX31_RS20490 ribosome biogenesis GTPase Der (Rahnella sp. WP5)
MIPVVALVGRPNVGKSTLFNRLTKTRDALVADFPGLTRDRKYGRAEIEGNEFIIVDTGGI
DGTEDGVETRMAGQSLLAIEEADIVLFMVDARAGLMPADQGIAQHLRSREKATFLVANKT
DGLDPDSATGDFYSLGLGDVYAIAASHGRGVTQLIEHVLIPFVGEKPEEVELTEEEANAA
YWAEQEATGDEIPEDEEDNFDPQSLPIKLAIVGRPNVGKSTLTNRILGEERVVVYDMPGT
TRDSIYIPMVRDEREYVLIDTAGVRKRGKVTETVEKFSVIKTLQAIEDANVVMLVIDARE
GISDQDLSLLGFILNSGRSLVIVVNKWDGMSEEARAQVKDQLDLRLGFVDFARIHFISAL
HGSGVGNLFESVNEAYTCATTRVSTSLLTRIMQMAADDHQPPLVNGRRVKLKYAHAGGYN
PPIVVIHGNQVKDLADSYKRYLMNYFRRSLKVMGTPIRIQFKEGENPYEGKKNSLSPHQQ
RKRRRLMQHLKNKS