Protein Info for EX31_RS20460 in Rahnella sp. WP5

Annotation: glycoside hydrolase family 31 protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 787 PF13802: Gal_mutarotas_2" amino acids 27 to 213 (187 residues), 115.7 bits, see alignment E=6.5e-37 PF01055: Glyco_hydro_31_2nd" amino acids 262 to 587 (326 residues), 282.5 bits, see alignment E=1e-87 PF21365: Glyco_hydro_31_3rd" amino acids 596 to 682 (87 residues), 86.6 bits, see alignment E=2.6e-28 PF17137: DUF5110" amino acids 700 to 767 (68 residues), 27.6 bits, see alignment E=4.9e-10

Best Hits

KEGG orthology group: K01187, alpha-glucosidase [EC: 3.2.1.20] (inferred from 99% identity to rah:Rahaq_1054)

Predicted SEED Role

"Alpha-glucosidase (EC 3.2.1.20)" in subsystem D-Galacturonate and D-Glucuronate Utilization or Maltose and Maltodextrin Utilization (EC 3.2.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.20

Use Curated BLAST to search for 3.2.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (787 amino acids)

>EX31_RS20460 glycoside hydrolase family 31 protein (Rahnella sp. WP5)
MKTLKHWELAGQDEAGVDLRVDGKHLFSLRVLEQGLIRVFIQRNGELALDRTWSIAPQND
APWEGRDRLSNEGFSVPGYQLEQGDDCLTLTTEALRVTVHQPLWLDWEYKNEAGVWQPLV
KDRKTGAYMLGVKNEASAHYQLREPQDGVYGLGEKAGDLNRSGRRFEMRNLDAMGYNAAS
TDPLYKHIPFTLTRRNGVSFGVFYDNLSNVWLDLGNELDNYHLPYRRFSAEAGDLDYYLF
VGPTSLDVTKKLVRLTGKTTFGPKWSLGYSGSTMYYTDAPDAQQQLMKFITLCQQHQIPC
DSFQLSSGYTSIGNKRYVFNWNYDKVPEPEVMSKAFLDAGIRLAANIKPCLLQDHPQYEQ
VAEQGLFIRDSESDVPERSVFWDDEGSHLDFTHPAAVRWWQKGVTQQLLEKGIGSTWNDN
NEYEVWDSDARCHGFGKSIAIKHIRPVMPLLMMRASFEAQQNFAPEIRPYLISRSGCSGM
QRYVQTWSGDNRTNWQTLRYNTRMGIGMSLSGLYNVGHDVGGFSGDKPDAELFVRWVQNG
VMHPRFTIHSWNDDHTVNEPWMYPQVTPLIRDAIRLRYRLLPYFYTLLWQAHADDEPMLR
PTFLDHEHDKNTFAETDDFLMGRDLLVASVVDEGQRERNVYLPDNGMGWCDFYTGEWFAG
GQTITLDAPLDRLPLLVRAGAALPQSCRLAHVEASKDMLRELHVYPAPGQATSSGMLFED
DGESHRWEQNHALWLNWDIASDDQRINITLTQRGDFKPAWKTLSIVLPQGETREVWVNGV
KTNNYNL