Protein Info for EX31_RS20455 in Rahnella sp. WP5

Annotation: exodeoxyribonuclease VII large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 457 PF13742: tRNA_anti_2" amino acids 10 to 103 (94 residues), 116.7 bits, see alignment E=6.9e-38 TIGR00237: exodeoxyribonuclease VII, large subunit" amino acids 11 to 354 (344 residues), 452.7 bits, see alignment E=6.3e-140 PF01336: tRNA_anti-codon" amino acids 30 to 103 (74 residues), 47 bits, see alignment E=3.1e-16 PF02601: Exonuc_VII_L" amino acids 126 to 440 (315 residues), 384.4 bits, see alignment E=7.6e-119

Best Hits

Swiss-Prot: 84% identical to EX7L_SERP5: Exodeoxyribonuclease 7 large subunit (xseA) from Serratia proteamaculans (strain 568)

KEGG orthology group: K03601, exodeoxyribonuclease VII large subunit [EC: 3.1.11.6] (inferred from 100% identity to rah:Rahaq_1055)

MetaCyc: 75% identical to exodeoxyribonuclease VII subunit XseA (Escherichia coli K-12 substr. MG1655)
Exodeoxyribonuclease VII. [EC: 3.1.11.6]

Predicted SEED Role

"Exodeoxyribonuclease VII large subunit (EC 3.1.11.6)" in subsystem DNA repair, bacterial (EC 3.1.11.6)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.6

Use Curated BLAST to search for 3.1.11.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (457 amino acids)

>EX31_RS20455 exodeoxyribonuclease VII large subunit (Rahnella sp. WP5)
MSLPSSPPIFTVSRLNQTVRQLLENEMGQVWLSGEISNFSQPSSGHWYFTLKDDRAQVKC
AMFRNTSRRTTFRPQNGQQVLVRATITLYEPRGDYQLIAESMQEAGDGLLQQKFDLLKQQ
LAAEGLFDAQFKQPLPSPAKCVGVITSASGAALHDVLNVLKRRDPSLPVIIYPTSVQGAD
APGQIVRAIELANAREEVDVLIVGRGGGSLEDLWSFNDERVARAIFASRIPIVSAVGHET
DVTIADFVADLRAPTPSAAAELISRNQLELLRQIQSQQQRLEMAMDYYLAQLTQRFTRLN
HRLQQQHPHLRLARQQTTLFKLRRRLDEAMQNQLRQMLRRTDRLQQRLTQQQPQPRIHRA
QQRVQQLQYRLQQAMSSQLTESRQRFGTACTQLEGVSPLATLARGYSVTTTPQGALLKKT
TQTAPGDTLKTRLADGWIESEVKSIVVEKKVRKRAVK