Protein Info for EX31_RS20395 in Rahnella sp. WP5

Annotation: hydroxyethylthiazole kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02110: HK
  • amino acids 17 to 259 (243 residues), 301.5 bits, E=2.3e-94
• TIGR00694: hydroxyethylthiazole kinase
  • amino acids 17 to 261 (245 residues), 284.8 bits, E=3e-89

Best Hits

Swiss-Prot: 70% identical to THIM_PECAS: Hydroxyethylthiazole kinase (thiM) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K00878, hydroxyethylthiazole kinase [EC: 2.7.1.50] (inferred from 99% identity to rah:Rahaq_1063)

MetaCyc: 64% identical to hydroxyethylthiazole kinase (Escherichia coli K-12 substr. MG1655)
Hydroxyethylthiazole kinase. [EC: 2.7.1.50]; 2.7.1.50 [EC: 2.7.1.50]; Choline kinase. [EC: 2.7.1.50, 2.7.1.32]

Predicted SEED Role

"Hydroxyethylthiazole kinase (EC 2.7.1.50)" in subsystem Thiamin biosynthesis (EC 2.7.1.50)

MetaCyc Pathways

• thiamine diphosphate salvage II (5/5 steps found)
• prenylated FMNH₂ biosynthesis (3/3 steps found)
• thiamine diphosphate salvage V (3/3 steps found)
• thiamine diphosphate salvage IV (yeast) (4/7 steps found)
• thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (4/8 steps found)
• superpathway of phospholipid biosynthesis II (plants) (10/28 steps found)
• plasmalogen biosynthesis I (aerobic) (1/16 steps found)

KEGG Metabolic Maps

• Glycerophospholipid metabolism
• Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.32 or 2.7.1.50

Protein Sequence (269 amino acids)

>EX31_RS20395 hydroxyethylthiazole kinase (Rahnella sp. WP5)
MNTRPTFLPGTLAATSLKQLRLSAPLVHCLTNDVVQSFTANVLLALGASPAMVVDPAEAA
QFGAIADALLVNVGTLTHPHADAMRAAIHAASQAGKPWVLDPVAVGGLSWRTEFCREIIT
LKPAAIRGNASEILALSGLSASGRGVDSGDDSLSALPAAQQLARETGAVVAVTGETDYVT
DGMRSWAVAGGHPMMTRVVGTGCALSAVVAAFVALPGDRLDNVAAACRVMSCAGERACAV
VKGPGSFTPEYLDNLYLMGEEWLNGEEMQ