Protein Info for EX31_RS20270 in Rahnella sp. WP5

Annotation: nucleoside hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01156: IU_nuc_hydro
  • amino acids 5 to 301 (297 residues), 349 bits, E=1.4e-108

Best Hits

Swiss-Prot: 68% identical to IUNH_LEIMA: Inosine-uridine preferring nucleoside hydrolase (NSNH) from Leishmania major

KEGG orthology group: K01239, purine nucleosidase [EC: 3.2.2.1] (inferred from 99% identity to rah:Rahaq_1087)

MetaCyc: 44% identical to pyrimidine-specific ribonucleoside hydrolase RihA (Escherichia coli K-12 substr. MG1655)
Uridine nucleosidase. [EC: 3.2.2.3]; 3.2.2.3 [EC: 3.2.2.3]

Predicted SEED Role

"Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1)" in subsystem Purine conversions or Queuosine-Archaeosine Biosynthesis (EC 3.2.2.1)

MetaCyc Pathways

• superpathway of pyrimidine ribonucleosides salvage (10/10 steps found)
• superpathway of purines degradation in plants (15/18 steps found)
• purine nucleotides degradation I (plants) (10/12 steps found)
• adenosine nucleotides degradation I (7/8 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• adenine and adenosine salvage II (2/2 steps found)
• guanine and guanosine salvage II (2/2 steps found)
• pyrimidine ribonucleosides salvage II (2/2 steps found)
• pyrimidine ribonucleosides salvage III (2/2 steps found)
• guanosine nucleotides degradation I (3/4 steps found)
• NAD salvage (plants) (5/11 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.1

Use Curated BLAST to search for 3.2.2.1 or 3.2.2.3

Protein Sequence (315 amino acids)

>EX31_RS20270 nucleoside hydrolase (Rahnella sp. WP5)
MVKKIILDCDPGHDDAIAMLLAHGNPEIELLAVTTVVGNQTLEKVTRNALAVARVINLTG
VPFAAGATRPLVRQVEVADEIHGESGLDGPVLPEAAITLEPVHAIDLIIDLIMSHPPKTI
TLVPTGGLTNIAMAVRKEPRIAERVKEVVLMGGGYHVGNWSAVAEFNIKIDPEAAHIVFN
EKWPLTMVGLDLTHQALATPEVAARIAAVGTRPAKFVGELLDFFGLSYKDRQGFDSPPVH
DPCAVAYVIDPNVMTVQKVPVDIELTGTLTLGMTVADFRVPAPPDCHTQVAVKLDHQRFW
DLIVDALERIGEVKY