Protein Info for EX31_RS20210 in Rahnella sp. WP5

Annotation: sulfate/thiosulfate ABC transporter ATP-binding protein CysA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 364 TIGR00968: sulfate ABC transporter, ATP-binding protein" amino acids 3 to 242 (240 residues), 415.2 bits, see alignment E=4e-129 PF00005: ABC_tran" amino acids 18 to 164 (147 residues), 136.9 bits, see alignment E=7.3e-44

Best Hits

Swiss-Prot: 84% identical to CYSA_YERPS: Sulfate/thiosulfate import ATP-binding protein CysA (cysA) from Yersinia pseudotuberculosis serotype I (strain IP32953)

KEGG orthology group: K02045, sulfate transport system ATP-binding protein [EC: 3.6.3.25] (inferred from 100% identity to rah:Rahaq_1099)

MetaCyc: 77% identical to sulfate/thiosulfate ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-19-RXN [EC: 7.3.2.5]; ABC-7-RXN [EC: 7.3.2.5, 7.3.2.3]; 7.3.2.3 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-478 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-479 [EC: 7.3.2.5, 7.3.2.3]

Predicted SEED Role

"Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25)" in subsystem Cysteine Biosynthesis (EC 3.6.3.25)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.25

Use Curated BLAST to search for 3.6.3.25 or 7.3.2.3 or 7.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (364 amino acids)

>EX31_RS20210 sulfate/thiosulfate ABC transporter ATP-binding protein CysA (Rahnella sp. WP5)
MSIEINGINKYFGRTKVLNDISLDIASGEMVALLGPSGSGKTTLLRIIAGLENQSAGHLS
FHGKDVTRLHARDRQVGFVFQHYALFRHMTVFDNIAFGLTVLPRRERPNAEAIKQKVTKL
LEMVQLGHLANRYPAQLSGGQKQRVALARALAVEPQILLLDEPFGALDAQVRKELRRWLR
QLHEELKFTSVFVTHDQEEAMEVADRVVVMSQGNIEQVGAPQEIWREPATRFVLEFMGEV
NKIHGEIRGSQLYVGAYHWPLDNPPLHQGVVDLFLRPWEMEITPHSTARCPLPVKVLEVS
PRGHFWQLIVQAEGWHDEPLSVVLSEIHGNSAPQRGDRFYVGGLNGRLYAGDQPLQPVAL
AKSA