Protein Info for EX31_RS20080 in Rahnella sp. WP5

Annotation: NupC/NupG family nucleoside CNT transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 395 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details transmembrane" amino acids 32 to 56 (25 residues), see Phobius details amino acids 67 to 80 (14 residues), see Phobius details amino acids 87 to 109 (23 residues), see Phobius details amino acids 166 to 186 (21 residues), see Phobius details amino acids 193 to 213 (21 residues), see Phobius details amino acids 241 to 263 (23 residues), see Phobius details amino acids 269 to 291 (23 residues), see Phobius details amino acids 334 to 357 (24 residues), see Phobius details amino acids 375 to 394 (20 residues), see Phobius details TIGR00804: nucleoside transporter, NupC family" amino acids 5 to 392 (388 residues), 445.2 bits, see alignment E=1.2e-137 PF01773: Nucleos_tra2_N" amino acids 10 to 82 (73 residues), 67.9 bits, see alignment E=1.3e-22 PF07670: Gate" amino acids 91 to 190 (100 residues), 58.1 bits, see alignment E=1.6e-19 PF07662: Nucleos_tra2_C" amino acids 194 to 391 (198 residues), 207.5 bits, see alignment E=2.9e-65

Best Hits

Swiss-Prot: 83% identical to NUPC_ECOL6: Nucleoside permease NupC (nupC) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K11535, nucleoside transport protein (inferred from 100% identity to rah:Rahaq_1121)

MetaCyc: 83% identical to nucleoside:H+ symporter NupC (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-108A; TRANS-RXN-108B; TRANS-RXN-108C; TRANS-RXN-108D; TRANS-RXN-108E; TRANS-RXN-108F; TRANS-RXN-108G; TRANS-RXN-108H; TRANS-RXN-108I; TRANS-RXN-476

Predicted SEED Role

"Nucleoside permease NupC" in subsystem Deoxyribose and Deoxynucleoside Catabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (395 amino acids)

>EX31_RS20080 NupC/NupG family nucleoside CNT transporter (Rahnella sp. WP5)
MSRILHFVLALAVVAALALLVSRDRKNIRLRFIVQLLVIEVLLAYFFLNSSVGLGFVKGF
SDLFEKLLGFAAEGTGFVFGGMSDKGLAFFFLKVLCPIVFISALIGILQHIRVLPFVIRI
IGTVLSKINGMGKLESFNAISSLILGQSENFIAYKDILSKMSEKRMYTMAATAMSTVSMS
IVGAYMTMLEPRFVVAALVLNMFSTFIVLSLINPYKVDSEEDLQIGNTHQGQSFFEMLGE
YILAGFKVAIIVAAMLIGFIALISCLNALFDVVFGITFQGVLGYVFYPFAWMMGVPSHEA
LQVGSIMATKLVSNEFVAMLDLQKVATELSPRGVGILSVFLVSFANFSSIGIVAGAIKGL
NEHQGNVVSRFGLKLLYGSTLVSVLSASIAGLVLA