Protein Info for EX31_RS19890 in Rahnella sp. WP5

Annotation: hydrogenase nickel incorporation protein HypB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 259 TIGR00073: hydrogenase accessory protein HypB" amino acids 47 to 258 (212 residues), 305.8 bits, see alignment E=7.4e-96 PF02492: cobW" amino acids 77 to 236 (160 residues), 85.9 bits, see alignment E=1.4e-28

Best Hits

Swiss-Prot: 57% identical to HYPB_RHILV: Hydrogenase maturation factor HypB (hypB) from Rhizobium leguminosarum bv. viciae

KEGG orthology group: K04652, hydrogenase nickel incorporation protein HypB (inferred from 99% identity to rah:Rahaq_1161)

Predicted SEED Role

"[NiFe] hydrogenase nickel incorporation-associated protein HypB" in subsystem NiFe hydrogenase maturation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (259 amino acids)

>EX31_RS19890 hydrogenase nickel incorporation protein HypB (Rahnella sp. WP5)
MCSTCGCASGELRIEGVSPEHTHHHSHDHDDSHDHHHGLPFAPRRLVDIEMDVLAKNNHI
AAHNREHFAAAGILALNLVSSPGSGKTTLLTSTLTRLAPYFDCAVIEGDQQTTHDADRIR
ATGVPAIQVNTGKGCHLDAQMVHDALHQLSPNNNSLLFIENVGNLVCPASFDLGELHKVA
VLSVTEGEDKPLKYPHMFAASRLMVLNKIDLLPYVSFDVEACIANARQVNPQIEVIRLSA
TTGEGMEQWLAWLEQQRCA