Protein Info for EX31_RS19745 in Rahnella sp. WP5

Annotation: heme lyase CcmF/NrfE family subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 657 transmembrane" amino acids 6 to 23 (18 residues), see Phobius details amino acids 44 to 63 (20 residues), see Phobius details amino acids 96 to 113 (18 residues), see Phobius details amino acids 125 to 145 (21 residues), see Phobius details amino acids 176 to 198 (23 residues), see Phobius details amino acids 213 to 232 (20 residues), see Phobius details amino acids 248 to 265 (18 residues), see Phobius details amino acids 275 to 295 (21 residues), see Phobius details amino acids 313 to 332 (20 residues), see Phobius details amino acids 352 to 375 (24 residues), see Phobius details amino acids 395 to 414 (20 residues), see Phobius details amino acids 426 to 443 (18 residues), see Phobius details amino acids 449 to 470 (22 residues), see Phobius details amino acids 491 to 511 (21 residues), see Phobius details amino acids 618 to 637 (20 residues), see Phobius details TIGR00353: cytochrome c-type biogenesis protein CcmF" amino acids 53 to 641 (589 residues), 926.7 bits, see alignment E=3.4e-283 PF01578: Cytochrom_C_asm" amino acids 89 to 296 (208 residues), 188.6 bits, see alignment E=1.1e-59 PF16327: CcmF_C" amino acids 316 to 637 (322 residues), 439 bits, see alignment E=1.1e-135

Best Hits

Swiss-Prot: 66% identical to CCMF_HAEIN: Cytochrome c-type biogenesis protein CcmF (ccmF) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K02198, cytochrome c-type biogenesis protein CcmF (inferred from 100% identity to rah:Rahaq_1199)

Predicted SEED Role

"Cytochrome c heme lyase subunit CcmF" in subsystem Biogenesis of c-type cytochromes or Copper homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (657 amino acids)

>EX31_RS19745 heme lyase CcmF/NrfE family subunit (Rahnella sp. WP5)
MMPELGTFALCLALGLAVLLSIYPQWGAARQDGRMMAMARPLSYGMFACIALAFIILVHA
FVVNDFTVAYVANNSNTHLPVYYRVAATWGAHEGSLLLWVLLLSCWTLAVALFSRRMPQD
AVSRVLAVMGMITGGFLLFILLTSNPFIRSLPNFPVDGSDLNPVLQDIGLIFHPPLLYMG
YVGFSVAFAFAIASLMAGRLDTAWARWSRPWTQAAWVFLTIGIVLGSAWAYYELGWGGWW
FWDPVENASFMPWLAGTALIHSLAVTEKRGTFKAWTVLLAITAFSLCLLGTFLVRSGVLV
SVHSFASDPARGMFILAYLVIVIGGSLLLYAFKGNSVRAPSRHELHSRESYLLGNNVLLV
AAMLVVLLGTLLPLIHKQLGLGSISIGEPFFNTMFTWLMAPLALLLGIGPLVRWRRDEPS
KLWRRLGVALVVTLVLSILLPWLLQDKIVGMTVVGMLMSVWVIVLTLMELHERATHRHSF
WRGLTKLSRSHWGMVLGHLGVAVTVIGIAFSQNYSVERDVRMKSGDTVDIHDYHFIFRDV
RDITGPNYTGGEANIDVTQNGKQVTTLHAEKRFYSVARSMMTEAAIDGNLARDLYAALGE
EMDDGSWAVRLYYKPFVRWIWLGGLFMAAGGVLCILDPRYRMSKKLKKQGLAEAEEA