Protein Info for EX31_RS19385 in Rahnella sp. WP5

Annotation: NADH-quinone oxidoreductase subunit L

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 615 transmembrane" amino acids 6 to 21 (16 residues), see Phobius details amino acids 23 to 23 (1 residues), see Phobius details amino acids 31 to 53 (23 residues), see Phobius details amino acids 87 to 109 (23 residues), see Phobius details amino acids 118 to 136 (19 residues), see Phobius details amino acids 142 to 161 (20 residues), see Phobius details amino acids 173 to 192 (20 residues), see Phobius details amino acids 212 to 231 (20 residues), see Phobius details amino acids 251 to 270 (20 residues), see Phobius details amino acids 282 to 303 (22 residues), see Phobius details amino acids 310 to 329 (20 residues), see Phobius details amino acids 334 to 357 (24 residues), see Phobius details amino acids 377 to 400 (24 residues), see Phobius details amino acids 415 to 438 (24 residues), see Phobius details amino acids 458 to 478 (21 residues), see Phobius details amino acids 498 to 516 (19 residues), see Phobius details amino acids 537 to 558 (22 residues), see Phobius details amino acids 594 to 614 (21 residues), see Phobius details signal peptide" amino acids 22 to 22 (1 residues), see Phobius details TIGR01974: proton-translocating NADH-quinone oxidoreductase, chain L" amino acids 2 to 612 (611 residues), 767.1 bits, see alignment E=9.8e-235 PF00662: Proton_antipo_N" amino acids 70 to 120 (51 residues), 73.9 bits, see alignment 7.8e-25 PF00361: Proton_antipo_M" amino acids 136 to 428 (293 residues), 314 bits, see alignment E=9.7e-98

Best Hits

Swiss-Prot: 82% identical to NUOL_ECOLI: NADH-quinone oxidoreductase subunit L (nuoL) from Escherichia coli (strain K12)

KEGG orthology group: K00341, NADH dehydrogenase I subunit L [EC: 1.6.5.3] (inferred from 100% identity to rah:Rahaq_1271)

MetaCyc: 82% identical to NADH:quinone oxidoreductase subunit L (Escherichia coli K-12 substr. MG1655)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]; 7.1.1.- [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (615 amino acids)

>EX31_RS19385 NADH-quinone oxidoreductase subunit L (Rahnella sp. WP5)
MNLLYLTILFPLIGFLLLAFSRGRWSENTSATIGVGSIGLAALTTIYVGINFLSQKAAGV
EVFNQHLWNWMQVGDFNIPVTLTLDGLSMTMLSVVTGVGFFIHMFASWYMRGEEGYSRFF
AYTNLFIASMVILVLADNLLLMYLGWEGVGLCSYLLIGFYYTHPANGAAAMKAFIVTRVG
DVFLAFALFILYRELGTLNFRELMILAPQKLAVGDTAITWATLMLLGGAVGKSAQLPLQT
WLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMAPEILHLVGIIGAVTLVLAGFA
ALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFHLMTHAFFKALLFLSSGSVILACH
HEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPIITAGFFSKDEILAGALANGHLNLMVA
GLVGAFMTSLYTFRMIFIVFHGEEKIKAHAGKGITHHLPLLVLLVLSTFIGAMIVPPLKG
VLPDTTELAHGSVLTLEITSGVIAIAGILLAAALYLGKRSLVNSIAKSAPGRFFTTWWFH
AWGFDWLYDMIFVKPYLLVAKLLQRDPLNSMMNLPALFARLGNRGLTLSENGQVRWYVAS
MGLGAVVVLTLLLVI