Protein Info for EX31_RS19290 in Rahnella sp. WP5

Annotation: DNA topoisomerase (ATP-hydrolyzing) subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 883 TIGR01063: DNA gyrase, A subunit" amino acids 8 to 840 (833 residues), 1299.1 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 32 to 507 (476 residues), 553.6 bits, see alignment E=3.4e-170 PF03989: DNA_gyraseA_C" amino acids 538 to 584 (47 residues), 40.8 bits, see alignment 1.2e-14 amino acids 588 to 637 (50 residues), 43.7 bits, see alignment 1.5e-15 amino acids 645 to 688 (44 residues), 37.4 bits, see alignment 1.4e-13 amino acids 692 to 737 (46 residues), 54.4 bits, see alignment 6.6e-19 amino acids 744 to 789 (46 residues), 39.7 bits, see alignment 2.6e-14 amino acids 793 to 839 (47 residues), 47.8 bits, see alignment 7.9e-17

Best Hits

Swiss-Prot: 88% identical to GYRA_PECCA: DNA gyrase subunit A (gyrA) from Pectobacterium carotovorum

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 76% identity to vsa:VSAL_I1767)

MetaCyc: 85% identical to DNA gyrase subunit A (Escherichia coli K-12 substr. MG1655)
5.99.1.3-RXN [EC: 5.6.2.2]

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.6.2.2 or 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (883 amino acids)

>EX31_RS19290 DNA topoisomerase (ATP-hydrolyzing) subunit A (Rahnella sp. WP5)
MSDLAREITPINIEEELKNSYLDYAMSVIVGRALPDVRDGLKPVHRRVLFAMNVLGNDWN
KAYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQPFSLRYMLVDGQGNFGSVDGDSAAAM
RYTEIRMSKIAHELLADLEKETVDFVPNYDGTEQIPSVMPTRIPNLLVNGSSGIAVGMAT
NIPPHNLTEVINGCLAYIEDENISIEGLMEHIPGPDFPTAAIINGRRGIEEAYRTGRGKI
YIRARAEVEADAKTGRETIIVHEIPYQVNKARLIEKIAELVKEKRVEGISALRDESDKDG
MRIVIEIKRDAVGEVVLNNLYSLTQLQTSFGINMVALHQGQPKILGLKEILSAFVRHRRE
VVTRRTIFELRKARDRAHILEALAIALANIDPIIELIRAAPTPAEAKAGLVARSWDLGNV
SAMLASAGDDAARPEWLEPQFGIHDGKYFLTEQQAQAILDLRLQKLTGLEHEKLLDEYKE
LLVQIAELLFILSNPDRLMEVIREELEAIRDQYNDPRRTEITANTSDINIEDLINQEDVV
VTLSHQGYVKYQPLTDYEAQRRGGKGKSAARIKEEDFIDRLLVANTHDTILMFSSRGRLY
WMKVYQLPEASRGARGRPIVNLLPLEPNERITAILPVREYEEGRHVFMATASGTVKKTAL
TDFSRPRSAGIIAINLNEGDELIGVDLTDGKDEVMLFSAAGKVVRFSEEAVRSMGRTATG
VRGIRLGEGDSVVSLIIPRGDGCILTVTQNGFGKRTESTEYPTKSRATQGVISIKVSERN
GPVIGAIQVDEADQIMMITDAGTLVRTRVSEVSTVGRNTQGVTLIRTADDENVVGLQRVA
EPVEDEELDGVATEAGELEAAQAADLPDDAESEDDQPADDEEE