Protein Info for EX31_RS19285 in Rahnella sp. WP5

Annotation: two-component system sensor histidine kinase RcsC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 953 transmembrane" amino acids 16 to 41 (26 residues), see Phobius details amino acids 318 to 341 (24 residues), see Phobius details PF00512: HisKA" amino acids 474 to 538 (65 residues), 62.8 bits, see alignment 5e-21 PF02518: HATPase_c" amino acids 585 to 695 (111 residues), 106.2 bits, see alignment E=2.6e-34 PF09456: RcsC" amino acids 713 to 804 (92 residues), 92.1 bits, see alignment E=3.3e-30 PF00072: Response_reg" amino acids 832 to 940 (109 residues), 98.3 bits, see alignment E=6.3e-32

Best Hits

Swiss-Prot: 61% identical to RCSC_ECOLI: Sensor histidine kinase RcsC (rcsC) from Escherichia coli (strain K12)

KEGG orthology group: K07677, two-component system, NarL family, capsular synthesis sensor histidine kinase RcsC [EC: 2.7.13.3] (inferred from 61% identity to ecp:ECP_2261)

MetaCyc: 61% identical to histidine kinase RcsC (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Two-component sensor protein RcsC (EC 2.7.3.-)" (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (953 amino acids)

>EX31_RS19285 two-component system sensor histidine kinase RcsC (Rahnella sp. WP5)
MKYLASFRTTLKISRYLFRVLALMLWTLGVLLTTFYVLNIFHNKESEIRDEDNLNYDLAQ
SYIKNSNDVMHSIKFMAENRLSASVNGLDIINGIISTKNTAPSYLSLFPESDCSQINDSY
RNSLASLGNMIHYWKDNFSDAYDLNRVFLISGESLCLVDFGISSEPFERESQEQLKSLHE
KIMQYRNSKSTDKENSLYWVSQSGSKDSGNLYVLTPIYVGNKLEALMGIQQNLRAEDLLN
PNSQHTAVTLLDEDDQPLLGISEDGHYSAAPGDYPQASSFFGYVNGYKDLVYKKPLTPSS
LSVVYSMPFNTVIERFKILIFNAVLLNIFSGLLLFILAWVFERKMFLPAEENAFRLEEHE
QFNHKIVASAPVGISILRISDGVNILSNELAHNYISMLTQEDRQRITRIICDQQTNFVDV
ITKNNNNIQLSFVHSRYRNEDVAICVLIDVSARVKMEESLQEMANAAEQASQSKSMFLAT
VSHELRTPLYGIIGNLDLLQTQGLPQGVDRLVTAMNNSSALLLKIISDILDFSKIESEQL
KIEPQEFSCREVITHIAGNYLPLVVKKHLGLYCFIEHNVPLSFNGDPVRLQQVISNILNN
AIKFTDTGCIVLNVRTCGNYLEFQVRDTGVGIPEREITRLFDPFFQVGTGVQRHFQGTGL
GLAICEKLINLMDGDIEVESEPGLGSSFTVRIPLYNATFPSGEPLDNWQDKTVWLDVRNA
YLESYLMQLMRYHGANVQRCEPELQSQREDVLITDTPLIHKGELAAQIEFSIGHIGPPVE
TRPGYWIHSTSTPLEITALLNRIYRLETTAEEGGSATPEAAVAQDYSDITLLVVDDHPIN
RRLLADQLGTMGYIVVTANDGIDALDAMRAHEIDIVLSDVNMPRLDGYGLTAKLRELGHT
LPVIGVTANALAEQRQRCLEAGMDNCLSKPVTLETLRDTLRYYSALVRRKRQS