Protein Info for EX31_RS19200 in Rahnella sp. WP5

Annotation: DEAD/DEAH box helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04851: ResIII
  • amino acids 4 to 156 (153 residues), 101.1 bits, E=1.3e-32
• PF00270: DEAD
  • amino acids 7 to 154 (148 residues), 49.6 bits, E=7.8e-17
• PF00271: Helicase_C
  • amino acids 244 to 345 (102 residues), 50.6 bits, E=4.3e-17

Best Hits

Swiss-Prot: 81% identical to RADD_ECOLI: Putative DNA repair helicase RadD (radD) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to rah:Rahaq_1309)

MetaCyc: 81% identical to putative DNA repair helicase RadD (Escherichia coli K-12 substr. MG1655)
Nucleoside-triphosphatase. [EC: 3.6.1.15, 3.6.1.5]

Predicted SEED Role

"ATP-dependent RNA helicase YejH"

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5

Protein Sequence (587 amino acids)

>EX31_RS19200 DEAD/DEAH box helicase (Rahnella sp. WP5)
MTVFTLRPYQQDAVDATLRHFRQHDEPAVIVLPTGAGKSLVIAELARVARGRVLVLAHVK
ELVAQNHSKYLSYGLEADIFAAGLNLKQSSGKVIFGSVQSVAPNLAEFSGEFSLLIVDEC
HRISDDDNSQYQQIIGHLKAQNPRLRLLGLTATPYRLGKGWIYQYHHQGIVRGDANSLFR
DCIYELPLRYMIKNGFLVPPERLDMPVVSYDFSRLQASSNGLFSEADLNDELRQQKRITP
HIISQIIEFAQDRKGVMIFASTVEHAGEIFGLLPKGEAALISAQTHAVERDRLITAFKAQ
QLRYLVNVSVLTTGFDAPHVDLIAILRPTESVSLYQQIVGRGLRLAPGKTDCLILDYAGN
PHDLFTPEVGSHKPAGDNKPVQVFCPACGFANIFWGKTTEDGTVIEHFGRRCQGVLEDDE
GNREQCDFRFRFKSCPHCGAENDIAARRCHQCQEILVDPDDMLKAALRLKDALVLRCGGM
NLQNGRDDKGEWLKITYYDEDGADVSERFRLTTPAQRMAFLHNFLRPHQRAPGVPLEWNT
AADIVAQQILLRHPDFVVARMKGQFWQVREKMFDYEGRYRRANELRG