Protein Info for EX31_RS19125 in Rahnella sp. WP5

Annotation: cyclic di-GMP phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 560 transmembrane" amino acids 54 to 73 (20 residues), see Phobius details amino acids 274 to 296 (23 residues), see Phobius details PF12792: CSS-motif" amino acids 83 to 269 (187 residues), 83.5 bits, see alignment E=1.5e-27 PF00563: EAL" amino acids 304 to 537 (234 residues), 218.3 bits, see alignment E=1.1e-68

Best Hits

Swiss-Prot: 43% identical to PDEN_ECOLI: Probable cyclic di-GMP phosphodiesterase PdeN (pdeN) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 99% identity to rah:Rahaq_1324)

Predicted SEED Role

"Rtn protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (560 amino acids)

>EX31_RS19125 cyclic di-GMP phosphodiesterase (Rahnella sp. WP5)
MAFFYACNKVYEVSVDFLTTLFFYFLCAKRAKHMGLKTALTHTVLPKHLYLKRSLLFSVL
FFILFIAVTLTVIKTNNLREQNEIAQRTAQFTADYLRQLTDTMHRIGLLTTEPCHSIAGE
LNRDVAFSIGVRDFQLVRNGIVFCSSASGNQHIPVNSTLHDLEFDNNMSISLQMGTPAVP
NRAVILIWQRNPGATDDGVIATLELTLSPYMLFAQPGSGYRGFALLVGESALISSQAGLI
KVSDLPRSHFTQVKIDNYPFSIRLYSPSLTRDNILFTFSGGLLLSMLVCALIYHSLLNRH
NVEREVLRGLKNKEFFVEYQPVIDSKSLKVTGVEALLRWQHPIEGPIPPDVFISYAENQG
LIVDLTQHMMALVAADAHTLQHAFPGPGKLSINISPHHMNKISFHDDVMNFIEALPENAF
QVVFEITERGMIDTQGALREFNWLRRHKIEIAIDDFGTGHSALIYLEKFTLDYLKIDRGF
VMSIDQNTLIAPVLDTVLKLTEELKIKTVAEGVETQMQAEYLRNHGVTYLQGYLYSKSLG
VPELLDFIRDFNEKEATWQI