Protein Info for EX31_RS19020 in Rahnella sp. WP5

Annotation: ATP-dependent DNA helicase DinG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 724 PF06733: DEAD_2" amino acids 194 to 263 (70 residues), 31.1 bits, see alignment E=3.7e-11 PF13307: Helicase_C_2" amino acids 533 to 688 (156 residues), 115.1 bits, see alignment E=7.4e-37

Best Hits

Swiss-Prot: 70% identical to DING_SALTY: ATP-dependent DNA helicase DinG (dinG) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03722, ATP-dependent DNA helicase DinG [EC: 3.6.4.12] (inferred from 100% identity to rah:Rahaq_1346)

MetaCyc: 69% identical to ATP-dependent DNA helicase DinG (Escherichia coli K-12 substr. MG1655)
RXN0-4261 [EC: 5.6.2.3]

Predicted SEED Role

"ATP-dependent helicase DinG/Rad3" in subsystem DNA repair, bacterial DinG and relatives

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12 or 5.6.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (724 amino acids)

>EX31_RS19020 ATP-dependent DNA helicase DinG (Rahnella sp. WP5)
MALSPAVKNQISQWYKALQQQIPDFISRAPQRQMIAEVAKAFSGDSARHLAIEAPTGVGK
TLSYLIPGIAVSREEEKPLIVSTANVALQDQIFSKDLPLLKKIIPDLKFTGAFGRGRYVC
PRNLAAMSNENAQGDLGLFLGDELAPSSGEEQKLCKTLETALSRYEWDGLRDHYQKTIDD
PLWIKISTDKANCLSRNCHYFRECPYFVARKEIESADVVVTNHALVMAALESESVLPPAK
DLLLVLDEGHHLPEVARDALEVEAEITAVWNNLQLDNFVRQIEQCLALFTPKNPPPLTHA
ERLHNHCAEMRELLLLIEQAVSQHLPPAEPEAEYRFELGVLPPELTEQCSRLFKLTDGLR
SLAEYMVNDLSEKTGKHDIMRLHRAILHMSRMQGYLETMSKLWRLAALDKASNAPIGKWV
TRSYFDNQTHLVFHCAGIRVSEQLEKMLWRKVPHVVITSATLRSLNSFSRLQEMSGLTEK
AGDRFVSLDSPFNHVEQGKLVIPQMTLEPVMANEAAHIQEMADFFRAELAKGGHTGQLVL
FASNRALQQFVALLPDLRLMLLVQGDQPRYRLVEEHRKRVAAGTTSVLIGLQSFAEGLDL
KGELLTQVHIHKIAFPPIDSPVILTEGEWLKTLKRYPFEVQSLPSASFNLIQQVGRLIRS
HECTGEIVIYDKRLLTKSYGARLLAALPVFPIEQREMPAPGSVKRPDILKTAKKDPVRLR
ARRR