Protein Info for EX31_RS18865 in Rahnella sp. WP5

Annotation: heavy metal sensor histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 478 transmembrane" amino acids 20 to 41 (22 residues), see Phobius details amino acids 176 to 197 (22 residues), see Phobius details TIGR01386: heavy metal sensor kinase" amino acids 14 to 470 (457 residues), 479.2 bits, see alignment E=7.4e-148 PF00672: HAMP" amino acids 195 to 247 (53 residues), 28.5 bits, see alignment 2.3e-10 PF00512: HisKA" amino acids 253 to 315 (63 residues), 49.2 bits, see alignment E=6.8e-17 PF02518: HATPase_c" amino acids 362 to 471 (110 residues), 81.6 bits, see alignment E=8.5e-27

Best Hits

KEGG orthology group: K07644, two-component system, OmpR family, heavy metal sensor histidine kinase CusS [EC: 2.7.13.3] (inferred from 99% identity to rah:Rahaq_1380)

Predicted SEED Role

"Heavy metal sensor histidine kinase" in subsystem Cobalt-zinc-cadmium resistance

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (478 amino acids)

>EX31_RS18865 heavy metal sensor histidine kinase (Rahnella sp. WP5)
MSDRQLTHLPKRAMSLTLRATLLFALIASMVVSAVGFYLYYSMDKELVRRADYQVSGRVQ
YFRHLLANEFPLSQLSRNPGLFENMLGNERDILTFRLSGEKLLINVNPSRLALPPVAAVP
DGQPLTLNTVHHLTAPDGTPVRFARAAVQIQDGRTVEITGAHFMTEESRLLQTFRWEIIG
AVLFAYFLIAALGYLVIRRGLRPLRNMAYEAAQIHPASLSTRLSSENAPQELQQLIYSFN
DMLDRLAEGYQRLTQFSADLAHEIRTPVGALMGHCQVALYQPRTVEEYETLLANNIEELE
RISRMVENILFLARASDARSVLTITSIDVSSEITRIQEYFEGLAEEREMTLHGQGEGTLH
ADAILFQRALSNLVANAVRYGREGSAIVLRAEPHAKGMNIRVISQGEPVPPEKLGKLFDR
FYRADASRSEGGSSSGLGLSIVQAIMALHQGSVSVTSSAQGETCFTLYFPASDRKLSQ