Protein Info for EX31_RS18845 in Rahnella sp. WP5

Annotation: 3-isopropylmalate dehydratase small subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00171: 3-isopropylmalate dehydratase, small subunit
  • amino acids 1 to 187 (187 residues), 205 bits, E=4.7e-65
• PF00694: Aconitase_C
  • amino acids 1 to 122 (122 residues), 101 bits, E=3.4e-33

Best Hits

Swiss-Prot: 63% identical to LEUD1_MANSM: 3-isopropylmalate dehydratase small subunit 1 (leuD1) from Mannheimia succiniciproducens (strain MBEL55E)

KEGG orthology group: K01704, 3-isopropylmalate/(R)-2-methylmalate dehydratase small subunit [EC: 4.2.1.33 4.2.1.35] (inferred from 100% identity to rah:Rahaq_1384)

MetaCyc: 42% identical to maleate hydratase small subunit (Pseudomonas alcaligenes NCIMB 9867)
Maleate hydratase. [EC: 4.2.1.31]

Predicted SEED Role

"3-isopropylmalate dehydratase small subunit (EC 4.2.1.33)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 4.2.1.33)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• L-isoleucine biosynthesis II (5/8 steps found)
• gentisate degradation II (1/4 steps found)
• 2,5-xylenol and 3,5-xylenol degradation (1/13 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Butanoate metabolism
• C5-Branched dibasic acid metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.33, 4.2.1.35

Use Curated BLAST to search for 4.2.1.31 or 4.2.1.33 or 4.2.1.35

Protein Sequence (202 amino acids)

>EX31_RS18845 3-isopropylmalate dehydratase small subunit (Rahnella sp. WP5)
MEAFTTLTGIAAPLPAANVDTDVIMPKQFLKGIDRNGLDRGVFFDLRFLADGSPDPAFIL
NQPQWQGTKFLLVGPNFGCGSSREHAVWGLKQLGIRALIGTTFAGIFFDNCQRNGVLTVQ
LDPAQWMQMAQAVIHGDKNEMTVDLSAQKIILQNGERIDFAIDELSKIALLNGLDAIGTT
LQFRDEISQFQQAHLQRNPWLA