Protein Info for EX31_RS18830 in Rahnella sp. WP5

Annotation: choline transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 684 transmembrane" amino acids 21 to 40 (20 residues), see Phobius details amino acids 60 to 79 (20 residues), see Phobius details amino acids 100 to 120 (21 residues), see Phobius details amino acids 149 to 171 (23 residues), see Phobius details amino acids 199 to 222 (24 residues), see Phobius details amino acids 237 to 258 (22 residues), see Phobius details amino acids 270 to 293 (24 residues), see Phobius details amino acids 324 to 343 (20 residues), see Phobius details amino acids 355 to 376 (22 residues), see Phobius details amino acids 408 to 428 (21 residues), see Phobius details amino acids 454 to 472 (19 residues), see Phobius details amino acids 484 to 502 (19 residues), see Phobius details PF02028: BCCT" amino acids 22 to 506 (485 residues), 599 bits, see alignment E=3e-184 TIGR00842: transporter, betaine/carnitine/choline transporter (BCCT) family" amino acids 60 to 506 (447 residues), 588.8 bits, see alignment E=3.4e-181

Best Hits

Swiss-Prot: 78% identical to BETT_ECOLI: High-affinity choline transport protein (betT) from Escherichia coli (strain K12)

KEGG orthology group: K02168, high-affinity choline transport protein (inferred from 81% identity to ebi:EbC_19780)

MetaCyc: 78% identical to choline:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-99

Predicted SEED Role

"High-affinity choline uptake protein BetT" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (684 amino acids)

>EX31_RS18830 choline transporter (Rahnella sp. WP5)
MAIADTTERKSEKPRDQLNKVVFFTSAGLILAFTLMTIFFTDLSGKWISLTLNWVSATFG
WYYMLAATLYIVFVVFIACSRFGSIKLGPEQSKPEFSMMSWAAMLFAAGIGIDLMFFSVA
EPVTQYMLPPEGNGQTLEAARQAMTWTLFHYGLTGWAMYALMGIALGYFSYRYNLPLTIR
SALYPIFGKRINGPIGHSVDIAAVVGTIFGIATTLGIGVVQLNYGLKVLFHIPENLTVQA
ALILLSVIMATVSVTSGVNKGIRILSELNVLLALGLILFLLFFGNTEFLLNALVLNIGDY
INRFMGMTLNTFAFDRPTEWMNSWTLFFWAWWVAWSPFVGLFLARISRGRTIRQFVFGTL
IIPFVFTLLWLSIFGNSALYQILHGNLDFANEVISHPERGVYSLLAQYPGFTLSASVATI
TGLLFYVTSADSGSLVLGNFTSKLADINNDAPNWLRIFWSVTIGVLTLGMLMTDGVSALQ
NTTVIMGLPFSFVIFFIMAGLYKSLRLEDYRRASTQQTLAPLPATSDAALNWKQRLSRVM
NYPGTRHTQKMMDTVCRPAMQEVAQELELRGAKVTFNEHAPQEDEKIGPLELQVALGEEQ
NFVYQIWPVRYAVPAFTYRARAGKSHYYRLETFLLEGTQGNDLMDFTKHQVINDILDQYE
RHLNFLHINREAPGNTLTFPEQPV