Protein Info for EX31_RS18615 in Rahnella sp. WP5

Annotation: NAD(P)-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 344 PF04321: RmlD_sub_bind" amino acids 1 to 191 (191 residues), 50.2 bits, see alignment E=7.1e-17 PF01370: Epimerase" amino acids 3 to 232 (230 residues), 115.9 bits, see alignment E=7e-37 PF00106: adh_short" amino acids 3 to 78 (76 residues), 26.7 bits, see alignment E=1.3e-09 PF01073: 3Beta_HSD" amino acids 4 to 256 (253 residues), 80.3 bits, see alignment E=4.5e-26 PF16363: GDP_Man_Dehyd" amino acids 4 to 321 (318 residues), 42.4 bits, see alignment E=2.2e-14 PF13460: NAD_binding_10" amino acids 7 to 171 (165 residues), 43.1 bits, see alignment E=1.6e-14 PF07993: NAD_binding_4" amino acids 55 to 173 (119 residues), 36.4 bits, see alignment E=1.2e-12

Best Hits

Swiss-Prot: 69% identical to YBJS_ECOLI: Uncharacterized protein YbjS (ybjS) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to rah:Rahaq_1432)

Predicted SEED Role

"Putative nucleotide di-P-sugar epimerase or dehydratase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (344 amino acids)

>EX31_RS18615 NAD(P)-dependent oxidoreductase (Rahnella sp. WP5)
MKVLVTGASGGLGRNAVEFLRNKGIKVRATGRNAAMGSLLQKMGAEFIHADLTTLVSAQA
KALLADIDTVWHCSGLMSPWGSEQEFELANVRATRRLGEWSAAYGVENFVHISSPAIYFD
FHHHRDIQEDFRPHRFANSFARSKAAGEQVIHTLALSNPQTHFTILRPQGIFGPHDNVMM
PRLLQMLKYGGTLMLPRGGEALVDMTYVENAVHAMWLATQSQATESGRAFNITNQQPRPL
CTLVKQLMENLDIKYRIRSVPYPLLDMMARGMERISKSSQKEPVLTHYSVAKLNFDLTLD
TQRAQKELGYVPVISLDQGIIRTADWLRDHGKLQGLHGIGPKRD