Protein Info for EX31_RS18500 in Rahnella sp. WP5

Annotation: dimethylsulfoxide reductase subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 815 transmembrane" amino acids 20 to 40 (21 residues), see Phobius details TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 15 to 41 (27 residues), 20.5 bits, see alignment (E = 4.4e-08) TIGR02166: anaerobic dimethyl sulfoxide reductase, A subunit, DmsA/YnfE family" amino acids 15 to 815 (801 residues), 1363.4 bits, see alignment E=0 PF04879: Molybdop_Fe4S4" amino acids 57 to 117 (61 residues), 57.4 bits, see alignment 1.7e-19 PF00384: Molybdopterin" amino acids 120 to 576 (457 residues), 270.3 bits, see alignment E=3.7e-84 PF01568: Molydop_binding" amino acids 696 to 807 (112 residues), 94.6 bits, see alignment E=5.7e-31

Best Hits

Swiss-Prot: 82% identical to DMSA_ECOLI: Dimethyl sulfoxide reductase DmsA (dmsA) from Escherichia coli (strain K12)

KEGG orthology group: K07306, anaerobic dimethyl sulfoxide reductase subunit A [EC: 1.8.99.-] (inferred from 82% identity to ecp:ECP_0908)

MetaCyc: 82% identical to dimethyl sulfoxide reductase subunit A (Escherichia coli K-12 substr. MG1655)
DIMESULFREDUCT-RXN [EC: 1.8.5.3]

Predicted SEED Role

"Anaerobic dimethyl sulfoxide reductase chain A (EC 1.8.5.3)" (EC 1.8.5.3)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.8.5.3

Use Curated BLAST to search for 1.8.5.3 or 1.8.99.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (815 amino acids)

>EX31_RS18500 dimethylsulfoxide reductase subunit A (Rahnella sp. WP5)
MSDLNSNKTDSGGGISRRDLVKGTTLAGIAAAVGGISLPFKSIRAEKLQAPAKSTDEKIV
WSACTVNCGSRCPLRMHVVDNEIRYVETDNTGNDNYEGLHQVRACLRGRSMRRRVYNPDR
LKYPMKRVGKRGEGKFKPISWDEAFDTLAGSLKDIIHRYGNEAIYLNYGTGTLGGTMTRS
WPPGKTLIARLMNCCGGYLNHYGDYSSAQIAAGLNYTYGGWADGNSPSDIENSKLVVLFG
NNPGETRMSGGGVTYYLEQARQRSNARMIIVDPRYTDTGAGREDEWVPIRPGTDGALAAA
LAHVMITENLVDQPFLDKYCVGYDEKTLPAGVPENAHYKAYILGKGPDGVAKTPEWAAKI
TGISVDKIVKLGREIGQAKPAFITQGWGPQRHANGEIATRAIAMLAILTGNVGVNGGNSG
AREGSYEIPFVRMPTLENPVQTSISMFLWTDAIVRGPQMTAMKDGVRGKDKLDVPIKFIW
NYAGNCLINQHSDINRTHDILQDDKQCEMIVVIDNHMTSSAKYADIILPDCTASEQMDFC
LDASCGNMDYVIFADQAITPRFQCKNIYEMTSELSRRMGVEQQFTEGRTQEEWLRHLYQQ
SREALPELPDFETFRKQGIFKKRDPDQHHVAYKAFRENPAANPLSTPSGKIEIYSAELAK
IAAEWELSKEDVIDPLPVYAAGFENYDDPLASKYPLQMTGFHYKARTHSTYGNVDVLKAA
CRQEMWINPMDAAQRGITNGDMIRIFNDRGEVHIAAKVTPRMMPGVVALGEGAWYSPDSK
KIDRAGSINVLTSQRPSPLAKGNPSHTNLVQVAKV