Protein Info for EX31_RS18065 in Rahnella sp. WP5

Annotation: intermembrane transport protein PqiB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 transmembrane" amino acids 23 to 43 (21 residues), see Phobius details PF02470: MlaD" amino acids 47 to 138 (92 residues), 49.6 bits, see alignment E=2e-17 amino acids 162 to 229 (68 residues), 40.2 bits, see alignment E=1.7e-14 amino acids 291 to 393 (103 residues), 40.2 bits, see alignment E=1.7e-14

Best Hits

Swiss-Prot: 67% identical to PQIB_ECOLI: Intermembrane transport protein PqiB (pqiB) from Escherichia coli (strain K12)

KEGG orthology group: K06192, paraquat-inducible protein B (inferred from 100% identity to rah:Rahaq_1545)

Predicted SEED Role

"Paraquat-inducible protein B" in subsystem Oxidative stress

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (550 amino acids)

>EX31_RS18065 intermembrane transport protein PqiB (Rahnella sp. WP5)
MAGNNMAEHDGTAKVEKIKRWSPVWIVPIVTALIGAWILFYHYSHQGPEVTLITTTAEGI
EGGKTTIKSRNVDVGKVESVTLSDDLQQVIIKARLNTGMEKMLRGDSVFWVVKPQIGREG
ISGLGTLLSGAYIQLQPGNKGGEKSEYKLDDSPPLASPDAKGIRVSLESERSGQLSPGDP
VLFRGFQVGTVETSHFDPKARNIKYQLFIRAPYDTLVTDNVRFWKDSGVNFDMSAAGMRV
EMGSLTTLFSGGVSFDIPNGWTQGNEAKNGDSYRLFDDQKSIQESLYTRHTDYVMFFSDS
IRGLQPGAPVEFRGIRLGTVAEVPYPMNVVKQQMSDDYRIPVLIRIEPDRFVQLLGSNFN
IEDHIRDGIAQGLRASMKSANLLTGAMYIDLDFYPNEKPWKGPLEIGGHKLLPTVSGGFA
QIQQKLMTTLDKINNLPIEPMMREATSTLAESQRTMKSTQKTIESLNAIVSSKEMKNLPQ
DMQQTLIQLNRSLKGFQPGSPAYGNLVGDMQSLDKTLRELQPVLKTLNQKSNALIFAAPG
QDDPQPKKAK