Protein Info for EX31_RS18050 in Rahnella sp. WP5

Annotation: Lon protease family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 588 PF13654: AAA_32" amino acids 132 to 221 (90 residues), 50.5 bits, see alignment E=2.2e-17 PF05362: Lon_C" amino acids 433 to 513 (81 residues), 37.3 bits, see alignment E=2.2e-13

Best Hits

Swiss-Prot: 54% identical to LONH_ECOLI: Putative Lon protease homolog (ycbZ) from Escherichia coli (strain K12)

KEGG orthology group: K04770, Lon-like ATP-dependent protease [EC: 3.4.21.-] (inferred from 100% identity to rah:Rahaq_1549)

Predicted SEED Role

"Putative protease La homolog (EC 3.4.21.-)" (EC 3.4.21.-)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (588 amino acids)

>EX31_RS18050 Lon protease family protein (Rahnella sp. WP5)
MTNNRLDWQLLLPDSAPYEAIFATAAQLAPVDFAGIQPRLENGLTLFCHPRSRVRFMTLK
AQENREYLSRITQVVSDLLPEPAEVTGNRYHIEGNKVTLLAAKTAEDAFAAKSTCQYREW
VEPEQLFGCVRIYKDQIQLEPGLVHQVNGGVLVLSIRTLIAQPLMWLRLKQMITEGQYHW
VSPDETTPLPVTVPPMPLDIRLILVGDRLSLGDLSDMEPELVDSSIYGEFEADLPLTEES
DMETWCGYVNTLADELEIPRLAADAWPEFLKMAIRHSTDQGSLTLDPVWLNTQLSEAALY
NEDELLSVKSLEAASGARAWRESYLQERMQDEIELGQIRIETEGEVVGQINGLSVLEYPG
HPRALGEPSRISCVVHAGDGEFTDVERKAELGGNLHAKGMMIMQAFLISELELDQPLPFS
ASIVFEQSYGEVDGDSASLAELCALISALSMQPINQQIAVTGSVDQFGNVQPIGGVNEKI
EGFFEVCQRRGLTGKQGVILPTSNVRNLCLHPQVLDAVREGQFHLWAVDNAAEALPILTG
LIYTNEEEAEEERPSLLGLIQQRIALATSQGSPRYPWPLRWLNWFNRS