Protein Info for EX31_RS17895 in Rahnella sp. WP5

Annotation: glutamine synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00120: Gln-synt_C
  • amino acids 136 to 469 (334 residues), 339.6 bits, E=1e-105

Best Hits

Swiss-Prot: 76% identical to PUUA_ECOLI: Gamma-glutamylputrescine synthetase PuuA (puuA) from Escherichia coli (strain K12)

KEGG orthology group: K09470, gamma-glutamylputrescine synthase [EC: 6.3.1.11] (inferred from 100% identity to rah:Rahaq_1578)

MetaCyc: 76% identical to glutamate-putrescine ligase (Escherichia coli K-12 substr. MG1655)
Glutamate--putrescine ligase. [EC: 6.3.1.11]

Predicted SEED Role

"Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11)" (EC 6.3.1.11)

MetaCyc Pathways

• superpathway of L-arginine and L-ornithine degradation (10/13 steps found)
• superpathway of ornithine degradation (6/8 steps found)
• putrescine degradation II (3/4 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (8/11 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.11

Protein Sequence (474 amino acids)

>EX31_RS17895 glutamine synthetase (Rahnella sp. WP5)
MNEIKKEISGTEPLAKHVEERSSAFQDEVSQYLERHPQTLHVDVLLTDLNGSFRGKRIPV
SALRKLEKGCYFPASVFAMDILGNVVEEAGLGQELGEPDRTCVPVPGTLTPSASDPEHTG
QLLLTMLDEDGTPFNVEPRNVLNHIWQTLRQRGLFPVVAVELEFYLLDRQRDAEGYIQPP
CTPGTQERSMHTQVYSVDNLDHFADVLSDIDRLAHIQGLPADGAVAESSPGQFEINLHHS
ENILRACDYALMLKRLVRLVAENHDMDATFMAKPYDEYAGSGMHIHLSVLDDNGKNLFAN
ASGEDSELMKRALAGMIALMPASMAVLAPNVNSFRRFQPGMYVPTQASWGHNNRTVALRV
PCSGTADHRVEYRVAGADANPYLVVATVLAGMLYGLDNDLPLPEPVTGNGLEQEGIPLPI
RQSDALYDFDDQPALKKILGERFSHVYHTCKSDELLQFERRVTETEIDWMLRNA