Protein Info for EX31_RS17845 in Rahnella sp. WP5

Annotation: cellulase family glycosylhydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 403 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF07745: Glyco_hydro_53" amino acids 43 to 402 (360 residues), 389.6 bits, see alignment E=1.1e-120

Best Hits

KEGG orthology group: K01224, arabinogalactan endo-1,4-beta-galactosidase [EC: 3.2.1.89] (inferred from 100% identity to rah:Rahaq_1589)

Predicted SEED Role

"Putative galactosidase"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.89

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (403 amino acids)

>EX31_RS17845 cellulase family glycosylhydrolase (Rahnella sp. WP5)
MTFFSKKIFNKSAMACVLLAVSFSTVAQEVVIKKVANVPADFIKGADISTLIDVEKHGGK
FFDQNHKQTDAMVILRQNGFNYVRLRLWVDPKDAQGHPYGGGNNDLATTLELAKRAKAQG
FKILLDFHYSDFWTDPGKQFKPKSWEKMDYPQLKTAIHDYSREVIARFRAQGTVPDMVQV
GNEMNGGILWPEGKSWGAGGGEFDRLAGLLNAAIAGVRADEKNPSDIKIMLHLAEGTKND
TFRWWFDEINQRKVPYDVIGLSFYTYWNGPVSALQTNMDDISKRYNKDVIVVEVAYGYTT
KNCDAAENSFTEKEAKDGGYPGTVQGQADFLSDMIKSLVAVPDHRGKGLFYWEPTWIAVD
GAGWATPAGMTYINDHWKEGNARENQALFSCDGQVLPSIKVFN