Protein Info for EX31_RS17370 in Rahnella sp. WP5

Annotation: glyoxylate/hydroxypyruvate reductase GhrA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02826: 2-Hacid_dh_C
  • amino acids 106 to 278 (173 residues), 140.4 bits, E=4.1e-45
• PF03446: NAD_binding_2
  • amino acids 140 to 227 (88 residues), 32.4 bits, E=9.3e-12

Best Hits

Swiss-Prot: 68% identical to GHRA_YERE8: Glyoxylate/hydroxypyruvate reductase A (ghrA) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K12972, gyoxylate/hydroxypyruvate reductase A [EC: 1.1.1.79 1.1.1.81] (inferred from 99% identity to rah:Rahaq_1691)

MetaCyc: 57% identical to glyoxylate/hydroxypyruvate reductase A (Escherichia coli K-12 substr. MG1655)
Glyoxylate reductase (NADP(+)). [EC: 1.1.1.79]; 1.1.1.- [EC: 1.1.1.79]

Predicted SEED Role

"Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81); 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (EC 1.1.1.-)" in subsystem Glycolate, glyoxylate interconversions (EC 1.1.1.-, EC 1.1.1.215, EC 1.1.1.26, EC 1.1.1.79, EC 1.1.1.81)

MetaCyc Pathways

• ketogluconate metabolism (8/8 steps found)
• L-serine biosynthesis II (3/4 steps found)
• formaldehyde assimilation I (serine pathway) (8/13 steps found)
• L-arabinose degradation IV (3/8 steps found)
• D-xylose degradation IV (2/7 steps found)
• superpathway of pentose and pentitol degradation (13/42 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Aminosugars metabolism
• Ascorbate and aldarate metabolism
• Benzoate degradation via CoA ligation
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of type II polyketide products
• Biosynthesis of unsaturated fatty acids
• Bisphenol A degradation
• Butanoate metabolism
• C21-Steroid hormone metabolism
• Fructose and mannose metabolism
• Glycine, serine and threonine metabolism
• Glyoxylate and dicarboxylate metabolism
• Insect hormone biosynthesis
• Linoleic acid metabolism
• Nucleotide sugars metabolism
• Pentose phosphate pathway
• Polyketide sugar unit biosynthesis
• Pyruvate metabolism
• Retinol metabolism
• Tetrachloroethene degradation

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.-, 1.1.1.215, 1.1.1.79, 1.1.1.81

Use Curated BLAST to search for 1.1.1.- or 1.1.1.215 or 1.1.1.26 or 1.1.1.79 or 1.1.1.81

Protein Sequence (313 amino acids)

>EX31_RS17370 glyoxylate/hydroxypyruvate reductase GhrA (Rahnella sp. WP5)
MNIIFYHPTSDAKPWIDGITKRLPQANVRAWKANDQDPADYALVWRPPHAMLAPRTMLKG
IFALGAGVDAILTQEQRQPGTLPAGVPLLRLEDTGMALQMQEYALATVLRYFRRMDEYQL
FQQQKQWRPLAPHSRSEFIIGILGAGVLGSSVAKALVDMQFTVRSWSRSAKSQEGVESFY
GDDQLAAFASGCKVLINLLPNTPHTAGVLNHKLFTNLSHNAYLINLGRGGHLVEGDLLRA
LDSGQIAAATLDVFVEEPLPGMHPFWSHPRVSITPHVGADTLPEEAMNSIVSNILAIEAG
REPTGRVDPGRGY