Protein Info for EX31_RS17285 in Rahnella sp. WP5

Annotation: carboxy-S-adenosyl-L-methionine synthase CmoA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00740: tRNA (cmo5U34)-methyltransferase
  • amino acids 4 to 242 (239 residues), 419.6 bits, E=2e-130
• PF00891: Methyltransf_2
  • amino acids 54 to 167 (114 residues), 28.1 bits, E=3.8e-10
• PF13847: Methyltransf_31
  • amino acids 57 to 164 (108 residues), 36.5 bits, E=1.2e-12
• PF13649: Methyltransf_25
  • amino acids 60 to 157 (98 residues), 48.5 bits, E=3.5e-16
• PF08242: Methyltransf_12
  • amino acids 62 to 159 (98 residues), 40.9 bits, E=8.6e-14
• PF08241: Methyltransf_11
  • amino acids 62 to 161 (100 residues), 38.9 bits, E=3.3e-13

Best Hits

Swiss-Prot: 90% identical to CMOA_YERE8: Carboxy-S-adenosyl-L-methionine synthase (cmoA) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K15256, tRNA (cmo5U34)-methyltransferase [EC: 2.1.1.-] (inferred from 100% identity to rah:Rahaq_1709)

MetaCyc: 82% identical to carboxy-S-adenosyl-L-methionine synthase (Escherichia coli K-12 substr. MG1655)
RXN0-7066

Predicted SEED Role

"tRNA (uridine-5-oxyacetic acid methyl ester) 34 synthase"

MetaCyc Pathways

• 5-(methoxycarbonylmethoxy)uridine biosynthesis (3/3 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Anthocyanin biosynthesis
• Benzoxazinone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Histidine metabolism
• Insect hormone biosynthesis
• Naphthalene and anthracene degradation
• Phenylpropanoid biosynthesis
• Porphyrin and chlorophyll metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Protein Sequence (247 amino acids)

>EX31_RS17285 carboxy-S-adenosyl-L-methionine synthase CmoA (Rahnella sp. WP5)
MANHDTLFSAPIDKLGDWTFDERVAEVFPDMIQRSVPGYSNIISMIGMLAERFVQPDSNV
YDLGCSLGAATLSMRRNIKVPGCEIIAVDNSPAMVERCRRHIDAFRADTPVSVIESDILD
IELKNASMVVLNFTLQFLEPADRQRLLNQVYQGMRPGAALVLSEKFSFEDKDVGELLFNM
HHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARLHQAGFQHTEVWFQCFNFGSLIA
LKAGEAQ