Protein Info for EX31_RS16630 in Rahnella sp. WP5

Annotation: phosphogluconate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 604 transmembrane" amino acids 133 to 151 (19 residues), see Phobius details amino acids 162 to 177 (16 residues), see Phobius details TIGR01196: phosphogluconate dehydratase" amino acids 5 to 603 (599 residues), 1047.9 bits, see alignment E=0 PF00920: ILVD_EDD_N" amino acids 68 to 379 (312 residues), 333.8 bits, see alignment E=1.1e-103 PF24877: ILV_EDD_C" amino acids 406 to 599 (194 residues), 183.8 bits, see alignment E=2.4e-58

Best Hits

Swiss-Prot: 79% identical to EDD_ECOLI: Phosphogluconate dehydratase (edd) from Escherichia coli (strain K12)

KEGG orthology group: K01690, phosphogluconate dehydratase [EC: 4.2.1.12] (inferred from 100% identity to rah:Rahaq_2351)

MetaCyc: 79% identical to phosphogluconate dehydratase (Escherichia coli K-12 substr. MG1655)
Phosphogluconate dehydratase. [EC: 4.2.1.12]

Predicted SEED Role

"Phosphogluconate dehydratase (EC 4.2.1.12)" in subsystem Entner-Doudoroff Pathway (EC 4.2.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (604 amino acids)

>EX31_RS16630 phosphogluconate dehydratase (Rahnella sp. WP5)
MINNTVARVTQRIINRSASTREAYLARMDAARSQTVHRAQLACGNLAHGFAACQPDEKNS
LKNMVRSDIAIITAYNDMLSAHQPYEHYPQRLKDALKSVGAVGQVAGGVPAMCDGVTQGQ
DGMELSLMSRDVIAMSAAIGLSHNMFDGALYLGICDKIVPGLVMAALSFGHLPAVFVPAG
PMSTGLPNKEKVRIRQLYTEGKVDRQALLEAEAASYHGIGTCTFYGTANTNQMVMEVMGL
HLPGASFVPPDCALRDALTDSAARQVTRLAETTGNYQPLGQMVNEKTVVNGIVALLATGG
STNLTMHMVAMARAAGIVINWDDFSELSDAVPSLCRIYPNGPADINQFQAAGGVQLVVRE
LLKNGLLHQDVNTVAGFGLDRYTQEPWLENGELAWREGAEHSLDTNVIASVQQPFEHHGG
TKVLSGNLGRAVMKTSAVPADNQIIEAPAVVFESQHDIVPAFEAGKLNRDCVVVVRFQGP
QANGMPELHKLMPPLGVLMDRGFRVALVTDGRLSGASGKVPSAIHVTPEAFSGGMLAKVR
DGDIIRVNGRTGELELRVDAAELAERKACQPDLSAEHIGCGRELFSALRSQLSGAEQGAC
CITF