Protein Info for EX31_RS16540 in Rahnella sp. WP5

Annotation: glycoside hydrolase family 1 protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 466 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF00232: Glyco_hydro_1" amino acids 2 to 463 (462 residues), 466.9 bits, see alignment E=2.8e-144

Best Hits

Swiss-Prot: 69% identical to ABGA_CLOLO: 6-phospho-beta-glucosidase (abgA) from Clostridium longisporum

KEGG orthology group: K01223, 6-phospho-beta-glucosidase [EC: 3.2.1.86] (inferred from 72% identity to crn:CAR_c02090)

MetaCyc: 59% identical to 6-phospho-beta-glucosidase B (Escherichia coli K-12 substr. MG1655)
3.2.1.M25 [EC: 3.2.1.M25]; 3.2.1.M25 [EC: 3.2.1.M25]; 6-phospho-beta-glucosidase. [EC: 3.2.1.M25, 3.2.1.86]

Predicted SEED Role

"6-phospho-beta-glucosidase (EC 3.2.1.86)" in subsystem Beta-Glucoside Metabolism (EC 3.2.1.86)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.86

Use Curated BLAST to search for 3.2.1.86 or 3.2.1.M25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (466 amino acids)

>EX31_RS16540 glycoside hydrolase family 1 protein (Rahnella sp. WP5)
MTFPRDFLWGGAVAANQCEGAWNIDGKGPSIQDVTPQGVMGPMTEQPTDNNLKLAGIDFY
HRYKEDIKLFAEMGFKVFRTSIAWSRIFPYGDEQTPNEAGLAFYDALFDECHKYGIEPLV
TLSHYETPLHLTKEYNGWANRAMIGFYTRYVTTVFTRYQHKVKYWLTFNEINSVLHHPLM
SGGIFTPKNELSQQDLYQAVHHELVASALATKIGREIMPQAKFGCMVLSLPIYPLTPNPD
DVIACMHADHRNDFFADVHVRGNYPGYMQRYFREQNIDLQMEEEDVQILTHTVDFLSFSY
YFSTCETVQTNVDGATGNLMGGVKNPHLAASEWGWQIDPQGLRYILNKYYDRYQLPLFIV
ENGLGAKDVLINEGAGEPTVNDDYRIQYLREHLEQVHEAIKDGVPVMGYTTWGCIDLVSA
STAQLSKRYGFIYVDRNDDGKGSLARYKKKSFYWYQKVIHSNGAEL