Protein Info for EX31_RS16505 in Rahnella sp. WP5

Annotation: sensor domain-containing phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 587 PF13185: GAF_2" amino acids 27 to 154 (128 residues), 39.2 bits, see alignment E=1.2e-13 PF01590: GAF" amino acids 27 to 154 (128 residues), 49.8 bits, see alignment E=8.1e-17 PF00563: EAL" amino acids 336 to 569 (234 residues), 226.8 bits, see alignment E=4e-71

Best Hits

KEGG orthology group: None (inferred from 74% identity to kpe:KPK_3392)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (587 amino acids)

>EX31_RS16505 sensor domain-containing phosphodiesterase (Rahnella sp. WP5)
MLTDLSSDESKKRAAINLLKSHDEVRDEALKKYTLLACQILNVPMCFVSVLDDEKHYIKS
AQNMTVTETSLGEAFCTNTLEQGETYICSDTHRHPDFKTYDAVTEAPFVRFYAGCPLKTQ
DGVAVGTLCLLDTHSRTLSDEQINIFEQLAGLVSGFLASWHNVGYIDIVTLLPNRQRMLK
DIAVSKESTFKLVIIDCIDMPFAYEIARSLGMTAVEHLLRDMVLQLQARLKLTQQLYAVA
VGRFAYFTHEKGNLPLENIALSLRGVQAKINPEVPLDLDIHIGDSGCCDNAMTPGEILRR
AVSALHEAISHGHRFMTYDEALDNRKKNDFSLLTGLREALHEDRGLYLVYQPKVSLVTGR
ITGVEALLRWKHAKHGEVLPGLFIPLVERTSLMRELTEWVIDHAIAQLSQWRDRRIFLPV
SINLAASDFSRPDFADELEQKIRNVGLTPASLGVECLETEKMLESPAALNGLDMLKLRGF
KISLDDFGSGYSNINYLRQIPMDIIKLDRSIISKIASDKASCIIVRNVVRLLKQLDYVVL
AEGVEDDKTVEILRALGCDEVQGYVYARPMSARDLEVWYHDRNESRA