Protein Info for EX31_RS15905 in Rahnella sp. WP5

Annotation: DNA polymerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 934 transmembrane" amino acids 767 to 778 (12 residues), see Phobius details TIGR00593: DNA polymerase I" amino acids 9 to 934 (926 residues), 935.4 bits, see alignment E=2.1e-285 PF02739: 5_3_exonuc_N" amino acids 9 to 170 (162 residues), 195.3 bits, see alignment E=1.2e-61 PF01367: 5_3_exonuc" amino acids 171 to 272 (102 residues), 107.2 bits, see alignment E=1.1e-34 PF01612: DNA_pol_A_exo1" amino acids 335 to 521 (187 residues), 137.3 bits, see alignment E=9.7e-44 PF00476: DNA_pol_A" amino acids 556 to 931 (376 residues), 549.4 bits, see alignment E=8.4e-169

Best Hits

Swiss-Prot: 77% identical to DPO1_ECOLI: DNA polymerase I (polA) from Escherichia coli (strain K12)

KEGG orthology group: K02335, DNA polymerase I [EC: 2.7.7.7] (inferred from 76% identity to dze:Dd1591_4164)

MetaCyc: 77% identical to DNA polymerase I (Escherichia coli K-12 substr. MG1655)
3.1.11.-; 3.1.11.-; DNA-directed DNA polymerase. [EC: 2.7.7.7]

Predicted SEED Role

"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (934 amino acids)

>EX31_RS15905 DNA polymerase I (Rahnella sp. WP5)
MAQIAENPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLNMLRSLLLQYTPSHVAV
VFDAKGKTFRDELFEQYKSHRPPMPDDLRSQIEPLHQMVKAMGLPLLVVSGVEADDVIGT
LALEAELAGHAVLISTGDKDMAQLVTPGVTLINTMNNAILGPDEVVEKYGVPPSLIIDFL
ALMGDSSDNIPGVPGVGEKTAQALLQGIGGLDALYADLDKVSTLSFRGAKTMAAKLADNK
DVAYLSYQLATIKTDVKLDLTCAELTVSEPDADELHKLFGRYEFKRWLADVESGTWLSGK
KSAGKAPAAAKKSAQAEDTTSAAVIEPNQLSQDGYVTILDQATLDEWIGYLKGSEVFAFD
TETDGLDTLTANLIGLSFATEAGKAAYLPVAHDYLDAPVQLDRDEVLKQLKPILEDEKQR
KVGQNLKFDRGVLLRYDIELKGIAFDTMLESYVLDSVSGRHDMDSLADRYLGHKTVSFEE
IAGKGKNQLTFNQIALEQAAPYAAEDADVTLQLHQALWPRIAESESLKTVFTDIEMPLVP
VLSHIERTGVLIDQNILAAHSKELAIRLNELENKAHELAEEPFNLSSTKQLQAILYEKQK
LPILKKTPGGAPSTNEEVLAELALNYPLPKVILEYRGLAKLKSTYTDKLPLMINPQSGRV
HTSYHQAVTATGRLSSSDPNLQNIPVRNDEGRRIRQAFIAPKDYIILAADYSQIELRIMA
HLSKDEGLLKAFAEGKDIHRATAAEVFGTPLEKVSGEQRRSAKAINFGLIYGMSAFGLSR
QLNIPRKESQRYMDLYFERYPGVLEYMERTRTQASEQGYVETLDGRRLYLPDIKSSNAMR
RKGAERAAINAPMQGTAADIIKRAMISVADWLKQPDAPRARIIMQVHDELVFEVHKDDVE
VASEKIRNLMEASMTLAVPLKVDVGTGENWDQAH