Protein Info for EX31_RS15820 in Rahnella sp. WP5

Annotation: ATP-dependent DNA helicase RecG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 693 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF17191: RecG_wedge" amino acids 15 to 171 (157 residues), 75.2 bits, see alignment E=1.9e-24 TIGR00643: ATP-dependent DNA helicase RecG" amino acids 28 to 664 (637 residues), 810 bits, see alignment E=8.8e-248 PF01336: tRNA_anti-codon" amino acids 63 to 135 (73 residues), 34.5 bits, see alignment E=6.3e-12 PF04851: ResIII" amino acids 272 to 430 (159 residues), 44.3 bits, see alignment E=7.3e-15 PF00270: DEAD" amino acids 273 to 436 (164 residues), 90.7 bits, see alignment E=3.7e-29 PF00176: SNF2-rel_dom" amino acids 297 to 435 (139 residues), 24.3 bits, see alignment E=6.6e-09 PF07517: SecA_DEAD" amino acids 297 to 363 (67 residues), 29.3 bits, see alignment E=2.1e-10 PF00271: Helicase_C" amino acids 479 to 587 (109 residues), 68.8 bits, see alignment E=1.9e-22 PF19833: RecG_dom3_C" amino acids 617 to 672 (56 residues), 38.3 bits, see alignment 4.7e-13

Best Hits

Swiss-Prot: 81% identical to RECG_ECO57: ATP-dependent DNA helicase RecG (recG) from Escherichia coli O157:H7

KEGG orthology group: K03655, ATP-dependent DNA helicase RecG [EC: 3.6.4.12] (inferred from 83% identity to eca:ECA0036)

Predicted SEED Role

"ATP-dependent DNA helicase RecG (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (693 amino acids)

>EX31_RS15820 ATP-dependent DNA helicase RecG (Rahnella sp. WP5)
MKGRLLDAIPLTSLSGVGASQADKLAKLGLETIQDLLLHLPLRYEDRTRLYTINDLQPGI
FATIEGEVLRSDISFGRRRMLTCQISDGTGMATLRFFNFNAAMKNSLATGRRVTAYGEIK
RGTIGAEIIHPEYRIQGENSEVVLQESLTPVYPTTEGIRQATLRKLTDQALELLDTVPVA
ELLPEELSRSLIPLPQALHLLHRPPPDIQLADLEKGHHPAQRRLIMEELLAHNLSMLAVR
AGTQSYKALPLHHDDRLKNQFLASLPFSPTGAQARVVAEIEQDLAKSYPMMRLVQGDVGS
GKTLVAALAALCAIAQGQQVGLMAPTELLAEQHANNFRQWFEPLGIQVGWLAGKQKGKAR
QAQQDAIASGQVSMVVGTHAIFQEQVLFSSLSLVIIDEQHRFGVHQRLALWEKGLQQGFH
PHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRSDIISRVKSACE
QENRQAYWVCTLIEESEMLEAQAAEATWEGLKEALPALNIGLVHGRMKAQEKQAVMQAFK
QGEVQLLVATTVIEVGVDVPNASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKT
PLSKTAQKRLQVLRDSNDGFVIAQQDLEIRGPGELLGTRQTGSAEFKVADLLRDQAMIPD
VQRIARYLQQQFPDHAKALIERWLPERVRYTNA