Protein Info for EX31_RS15650 in Rahnella sp. WP5

Annotation: HlyD family secretion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 transmembrane" amino acids 45 to 68 (24 residues), see Phobius details PF00529: CusB_dom_1" amino acids 71 to 398 (328 residues), 29 bits, see alignment E=2e-10 PF13533: Biotin_lipoyl_2" amino acids 86 to 123 (38 residues), 31.8 bits, see alignment 2.5e-11 amino acids 280 to 309 (30 residues), 28.5 bits, see alignment (E = 2.6e-10) PF16576: HlyD_D23" amino acids 237 to 360 (124 residues), 38.2 bits, see alignment E=2.4e-13 PF13437: HlyD_3" amino acids 281 to 377 (97 residues), 51.9 bits, see alignment E=2.8e-17

Best Hits

Swiss-Prot: 47% identical to MCHE_ECOLX: Microcin H47 secretion protein MchE (mchE) from Escherichia coli

KEGG orthology group: K02022, (no description) (inferred from 99% identity to rah:Rahaq_2738)

Predicted SEED Role

"COG0845: Membrane-fusion protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (448 amino acids)

>EX31_RS15650 HlyD family secretion protein (Rahnella sp. WP5)
MCCYLSKILFLQPVFLERLNMLFRKEVNEHQQSHWAGKALLLAGWPLWIVTTLTALFLFA
LLIFLIFANYTRRINVSGEIITQPHSINLFSPEQGVVTKLYVDTGKPVKKGQPLYEIDVS
RVTQAGNVSTTTLAAIKKQREQIDSIIAQLEHNKQETLQNLQQQLDQYQKAHEVSQSMVD
SAQEGLDAMKKSMQNYGAYQKKGLINTDQLNNQRYLFYQQQTSFQSLNTQAIQEALQITN
LRSELVTRAAEFDNQISQYGYQRNDLERQLAQADAKGSLLITAPTSGKISSLSVTPGQMV
NAGDSLAQLVPASNSPFFLVAWLPNESVPYVKAGEHINIRYEAYPFEKYGQFPGKVESIS
SAPVSEQELNSYASAPRTANGTVSGPYYKVIVSLDKKAMNWHGETLNLSSGMKAESTLFL
EKRPLYQWMLSPYYSMKKSVVGPINESR