Protein Info for EX31_RS15385 in Rahnella sp. WP5

Annotation: nitrogen fixation protein NifM

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 264 TIGR02933: nitrogen fixation protein NifM" amino acids 8 to 262 (255 residues), 402.4 bits, see alignment E=5.5e-125 PF13145: Rotamase_2" amino acids 109 to 237 (129 residues), 40.7 bits, see alignment E=5.8e-14 PF13616: Rotamase_3" amino acids 131 to 221 (91 residues), 47.7 bits, see alignment E=3.3e-16 PF00639: Rotamase" amino acids 135 to 224 (90 residues), 64.6 bits, see alignment E=2.1e-21

Best Hits

KEGG orthology group: K03769, peptidyl-prolyl cis-trans isomerase C [EC: 5.2.1.8] (inferred from 48% identity to ddd:Dda3937_02176)

Predicted SEED Role

No annotation

Isozymes

Compare fitness of predicted isozymes for: 5.2.1.8

Use Curated BLAST to search for 5.2.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (264 amino acids)

>EX31_RS15385 nitrogen fixation protein NifM (Rahnella sp. WP5)
MSMPVWHRYSRWKLAKQMKLSEELNPTPAQYQQFIEAWQRQQVIEEAVAQAALKHALNIE
PQSVEVTRQALAAELPELELTATETEELLLHQTLLREQLNWVQQQAPLPDDAQVEAWYRG
HAQHFVRPEQRYTRHLLLTVEGNSSAVREQIDAIARRLRDGHALFARQALRYSHCPSAMG
GGVLGWVGRGILYPQLEDTLFRLEAGQLSGAVETELGWHLLLCEQIRLPQPLPKAEALTR
VRQQLIARQQKHYQRQWLQQLINA