Protein Info for EX31_RS15360 in Rahnella sp. WP5

Annotation: Fe-S cluster assembly protein NifU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 275 TIGR02000: Fe-S cluster assembly protein NifU" amino acids 1 to 274 (274 residues), 449.1 bits, see alignment E=3.6e-139 PF01592: NifU_N" amino acids 4 to 123 (120 residues), 155.8 bits, see alignment E=9.6e-50 PF04324: Fer2_BFD" amino acids 135 to 184 (50 residues), 62.4 bits, see alignment 6.1e-21 PF01106: NifU" amino acids 208 to 272 (65 residues), 68.6 bits, see alignment E=6.4e-23

Best Hits

Swiss-Prot: 75% identical to NIFU_KLEPN: Nitrogen fixation protein NifU (nifU) from Klebsiella pneumoniae

KEGG orthology group: K13819, NifU-like protein (inferred from 79% identity to ddd:Dda3937_02171)

Predicted SEED Role

"Iron-sulfur cluster assembly scaffold protein NifU" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (275 amino acids)

>EX31_RS15360 Fe-S cluster assembly protein NifU (Rahnella sp. WP5)
MWNYSEKVKEHFFNPRNAKVVDEANAIGDVGSLSCGDALRLMLKVDPETEIILDAGFQTF
GCGSAIASSSALTELIIGRSLTQAQQVSNQQIADYLDGLPPEKMHCSVMGQEALRAAIRY
YRGEAPDEDHEEGALICKCFAVDEAQIRRAVLANGLTTLEDVINYTKAGGGCTSCHEKIE
LCLEEILKEHPVKNPVAAKVRDENWLAVEEVVESLRPHIRADGGDMSLVRVDGQDVTVAL
SGSCSGCMMTDMTLAWLQQKLVERMGRYMRVVAAA