Protein Info for EX31_RS15340 in Rahnella sp. WP5

Annotation: nitrogenase iron-molybdenum cofactor biosynthesis protein NifE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 457 TIGR01283: nitrogenase MoFe cofactor biosynthesis protein NifE" amino acids 3 to 456 (454 residues), 677.2 bits, see alignment E=5.1e-208 PF00148: Oxidored_nitro" amino acids 37 to 442 (406 residues), 317 bits, see alignment E=8.6e-99

Best Hits

Swiss-Prot: 79% identical to NIFE_KLEPN: Nitrogenase iron-molybdenum cofactor biosynthesis protein NifE (nifE) from Klebsiella pneumoniae

KEGG orthology group: K02587, nitrogenase molybdenum-cofactor synthesis protein NifE (inferred from 81% identity to dda:Dd703_0497)

MetaCyc: 67% identical to Nitrogenase iron-molybdenum cofactor biosynthesis protein NifE (Azotobacter vinelandii)
RXN-17026

Predicted SEED Role

"Nitrogenase FeMo-cofactor scaffold and assembly protein NifE" in subsystem Nitrogen fixation

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (457 amino acids)

>EX31_RS15340 nitrogenase iron-molybdenum cofactor biosynthesis protein NifE (Rahnella sp. WP5)
MKAKEILALIDEPACEYNHKQKSGCSLPKPGATAGGCAFDGAQIALLPVADVAHLVHGPI
GCTGSSWDNRGSRSSGPSINRMGFTTDMSEQDVIMGRGERRLFHAVQHIVSHYHPVAVFI
YNTCVPAMEGDDVEAVCRAASAAAGVPVISVDAAGFYGSKNLGNRIAGDVMVKKVIGQRE
PASWPENSPIPAGHRHSISLIGEFNIAGEFWHVLPLLDELGIRVLCSLSGDSRFAEIQTM
HRGEANMLVCSRALINVARKMEERYQIPWFEGSFYGLRSIADSLRTIAVLLKDPDLQART
ERLIEREEAATHLALAPYRTRLSGRKALLYTGGVKSWSVVSALQDLGITVVATGTRKSTE
EDKQRIRELMGEDVLMFDEGNARTLLDTLYRFGGDIMIAGGRNMYTAYKARLPFLDINQE
REHAFAGYHGLVNLAEQLCITLESPVWAQVNRLAPWR