Protein Info for EX31_RS15315 in Rahnella sp. WP5

Annotation: nitrogenase iron protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 291 PF00142: Fer4_NifH" amino acids 4 to 275 (272 residues), 436.2 bits, see alignment E=1.3e-134 PF02374: ArsA_ATPase" amino acids 4 to 47 (44 residues), 25.9 bits, see alignment 1.4e-09 TIGR01287: nitrogenase iron protein" amino acids 4 to 278 (275 residues), 470 bits, see alignment E=1.1e-145 PF01656: CbiA" amino acids 8 to 228 (221 residues), 50.6 bits, see alignment E=4.7e-17 PF10609: ParA" amino acids 9 to 60 (52 residues), 28.6 bits, see alignment 2.5e-10 PF13614: AAA_31" amino acids 10 to 74 (65 residues), 42.5 bits, see alignment E=1.8e-14

Best Hits

Swiss-Prot: 93% identical to NIFH_KLEP3: Nitrogenase iron protein (nifH) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: K02588, nitrogenase iron protein NifH [EC: 1.18.6.1] (inferred from 94% identity to dda:Dd703_0491)

MetaCyc: 93% identical to [FeMo]-nitrogenase complex nitrogenase reductase component monomer (Klebsiella pneumoniae)

Predicted SEED Role

"Nitrogenase (molybdenum-iron) reductase and maturation protein NifH" in subsystem Nitrogen fixation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.6.1

Use Curated BLAST to search for 1.18.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (291 amino acids)

>EX31_RS15315 nitrogenase iron protein (Rahnella sp. WP5)
MAMRQCAIYGKGGIGKSTTTQNLVAALAEMNKKVMIVGCDPKADSTRLILHAKAQNTIME
MAAEVGSVEDLELEDVMQIGYGGVRCAESGGPEPGVGCAGRGVITAINFLEEEGAYVPDL
DFVFYDVLGDVVCGGFAMPIRENKAQEIYIVCSGEMMAMYAANNISKGIVKYAKSGKVRL
AGLICNSRQTDREDELIIALAEKLGTQMIHFVPRDNIVQRAEIRRMTVIEYDPTCAQADQ
YRALANKIVNNTKMVVPTPVTMDELEALLMEFGIMEEEDLTIVGRTAAEEA