Protein Info for EX31_RS15085 in Rahnella sp. WP5

Annotation: lipid III flippase WzxE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 416 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 45 to 66 (22 residues), see Phobius details amino acids 79 to 105 (27 residues), see Phobius details amino acids 117 to 135 (19 residues), see Phobius details amino acids 147 to 169 (23 residues), see Phobius details amino acids 176 to 195 (20 residues), see Phobius details amino acids 216 to 238 (23 residues), see Phobius details amino acids 258 to 278 (21 residues), see Phobius details amino acids 299 to 322 (24 residues), see Phobius details amino acids 334 to 355 (22 residues), see Phobius details amino acids 362 to 382 (21 residues), see Phobius details amino acids 393 to 412 (20 residues), see Phobius details PF01943: Polysacc_synt" amino acids 4 to 283 (280 residues), 45.7 bits, see alignment E=6.2e-16 PF13440: Polysacc_synt_3" amino acids 83 to 330 (248 residues), 32.4 bits, see alignment E=6e-12

Best Hits

Swiss-Prot: 74% identical to WZXE_SHIFL: Lipid III flippase (wzxE) from Shigella flexneri

KEGG orthology group: K03328, polysaccharide transporter, PST family (inferred from 97% identity to rah:Rahaq_4256)

MetaCyc: 74% identical to lipid IIIECA flippase (Escherichia coli K-12 substr. MG1655)
7.5.99.a [EC: 7.5.99.a]

Predicted SEED Role

"WzxE protein"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.5.99.a

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (416 amino acids)

>EX31_RS15085 lipid III flippase WzxE (Rahnella sp. WP5)
MSLAKASVWTAGSTLIKIGAGLLVVKLLAVAFGPDGVGQAGNFRQLVTVLGVLSGAGIFN
GVTKYVAEHHENPERLKAVLGTSSTLILGFSTLLAAIFLLAAGPISVGLFGHDRYQSVVR
AVAFIQMGIAYANYFQAILKGYRDAMGNALAVIGGSLIGLLAYLLCFKLGGYEGALAGLA
LVPALVILPAGGMVWRRKSFPLAYLKPGWDRFIASHLGKFTLMALITSVTLPVAYVMMRN
LLAEHYSWKDVGIWQGVSSISDAYLQFITASFSVYLLPTLSRLTDKRDVTREIVKSLKFV
LPAVAAVSFTVWLLRDFAIWLLFSKQFIAMRDLFAWQLVGDVLKVGSYVFGYLVIARASL
RFYVLTEISQFALLTGFSHWLIPIRGSLGAAQAYMATYVVYFALCCTVFVIYRRRA