Protein Info for EX31_RS15030 in Rahnella sp. WP5

Annotation: DNA helicase Rep

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 674 TIGR01074: ATP-dependent DNA helicase Rep" amino acids 2 to 665 (664 residues), 1180.7 bits, see alignment E=0 PF00580: UvrD-helicase" amino acids 3 to 266 (264 residues), 311.8 bits, see alignment E=1.5e-96 PF13245: AAA_19" amino acids 7 to 249 (243 residues), 90.2 bits, see alignment E=4.1e-29 PF13361: UvrD_C" amino acids 271 to 613 (343 residues), 264.5 bits, see alignment E=5.8e-82 PF13538: UvrD_C_2" amino acids 554 to 608 (55 residues), 31.6 bits, see alignment 3.5e-11

Best Hits

Swiss-Prot: 86% identical to REP_SALTY: ATP-dependent DNA helicase Rep (rep) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03656, ATP-dependent DNA helicase Rep [EC: 3.6.4.12] (inferred from 89% identity to eca:ECA4215)

MetaCyc: 85% identical to ATP-dependent DNA helicase Rep (Escherichia coli K-12 substr. MG1655)
RXN-11135 [EC: 5.6.2.4]

Predicted SEED Role

"ATP-dependent DNA helicase Rep" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12 or 5.6.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (674 amino acids)

>EX31_RS15030 DNA helicase Rep (Rahnella sp. WP5)
MRLNPSQQHAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRECGYQARHIAAVTFTNKAA
REMKERVAQTLGRKEARGLLIATFHTLGLEIIKREFAALGMKSNFSLFDDQDQLALLKEL
SEKWLENDKTLLQQLVSTISNWKNDLVDPSHAAGLARSERDQIFAHCYSLYHDHMRACNI
LDFDDLILLPTLLLQSNEEVRERWQNKLRYLLVDEYQDTNTSQYMMVKLLVGNRARFTVV
GDDDQSIYSWRGARPQNLVLLKEDFPQLQVIKLEQNYRSTQRILKAANILIANNPHVFEK
RLFSELAYGETLKVVTANNEDHESERVVGELIAHHFINKTAYGDYAILYRGNHQSRLFEK
MLMQNRIPYRISGGTSFFSRPEIKDLLAYLRVLTNPDDDSAFLRIVNTPRREIGAATLQK
LGEWANQRNKGLFKASFDMGLSQSLTGRGLESLQRFTHWMGNISELAEREPVAAVRDLIH
GVDYESWLFETSPSPKAAEMRMKNVNQLFSWMTEMLEGTDLDEPMTLTQVVTRFTLRDMM
ERGENDEELDQVQLMTLHASKGLEFPYVYLVGMEEGLLPHQSSIDEDNVDEERRLAYVGI
TRAQKELTFTLCRERRQYGELIRPEPSRFLLELPQDDLAWESERKVVSAQERMVKGQSHL
EKIREQLAKAKGGN