Protein Info for EX31_RS14850 in Rahnella sp. WP5

Annotation: zinc/cadmium/mercury/lead-transporting ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 784 transmembrane" amino acids 175 to 191 (17 residues), see Phobius details amino acids 198 to 215 (18 residues), see Phobius details amino acids 235 to 259 (25 residues), see Phobius details amino acids 399 to 420 (22 residues), see Phobius details amino acids 429 to 455 (27 residues), see Phobius details amino acids 735 to 753 (19 residues), see Phobius details amino acids 758 to 779 (22 residues), see Phobius details PF00403: HMA" amino acids 97 to 153 (57 residues), 40.7 bits, see alignment 4.9e-14 TIGR01525: heavy metal translocating P-type ATPase" amino acids 230 to 778 (549 residues), 571.1 bits, see alignment E=4.8e-175 TIGR01494: HAD ATPase, P-type, family IC" amino acids 253 to 754 (502 residues), 257.3 bits, see alignment E=3.3e-80 PF00122: E1-E2_ATPase" amino acids 281 to 459 (179 residues), 151 bits, see alignment E=5.2e-48 PF00702: Hydrolase" amino acids 476 to 689 (214 residues), 117.1 bits, see alignment E=2.8e-37 PF08282: Hydrolase_3" amino acids 660 to 722 (63 residues), 28.6 bits, see alignment 2.4e-10

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 98% identity to rah:Rahaq_4202)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (784 amino acids)

>EX31_RS14850 zinc/cadmium/mercury/lead-transporting ATPase (Rahnella sp. WP5)
MIPLQKNNTCRCGCQGKHAPALRINKIGAIAQPGTHPAGCCSEKTPLAVSACSTTDSCCA
PKTGEHSHTSDDGNAEGSPGEDEPASPLSGSQQYSWKVQGMDCPACARKIEKAVSGIDGI
SHAKVLFATEKLVIDAGFDITQPVQNAVTAAGFTLQSLDKPQEKPTAEPSRLQEALPLIA
LSVLMLLSWVLDHFNPQLGKIAFIATTLVGLWPVLRQAIRQTRAGSPFAIETLMSIAAIG
ALFIGATAEAAMVLLLFMVGERLEAFASSRARSGVKALMALIPDTAVRIVNGKSETVPVS
SLRPGDIIEIAAGARLPADAVLQIAASFDESALTGESVPVDHLPGEKVPAGSLCVDRLAR
LEVASAPGNSAIDRILQLIEEADERRAPIERFLDTFSRYYTPVIMLMSLLVIIVPPLAMG
LSWETWIYRGLTLLLIGCPCALVISTPAAITSGLAAATRQGALIKGGAALEQLALIQTVA
LDKTGTLTEGKPEVTDVLLLNGASEGEVLSLASAVEEGSHHPLAQAILARAKTSNPLPLT
AQDRRALAGSGVEGLVSGRKIQVLAPKSVAENSLTAETLAQITDWESTGKTVVVVLQEEN
AIGLIAMQDRLRDDAAEALQKLKALGVSAVMLTGDNPRAAKTIADRLGIDFRAGLLPADK
VSAVTALNALKPVAMVGDGINDAPAMKAATIGIAMGSGTDVALETADAALTHNRLSGLAQ
MISLSRATRANIRQNITIALGLKAIFLVTTLMGLTGLWLAVLADSGATALVTANALRLLR
NKSK