Protein Info for EX31_RS14395 in Rahnella sp. WP5

Annotation: ADP-glyceromanno-heptose 6-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 310 PF04321: RmlD_sub_bind" amino acids 2 to 173 (172 residues), 29.8 bits, see alignment E=9.9e-11 PF01370: Epimerase" amino acids 2 to 236 (235 residues), 150.2 bits, see alignment E=2e-47 TIGR02197: ADP-glyceromanno-heptose 6-epimerase" amino acids 2 to 308 (307 residues), 450 bits, see alignment E=2.2e-139 PF01073: 3Beta_HSD" amino acids 3 to 220 (218 residues), 51 bits, see alignment E=3.2e-17 PF16363: GDP_Man_Dehyd" amino acids 3 to 253 (251 residues), 86.3 bits, see alignment E=8.6e-28

Best Hits

Swiss-Prot: 90% identical to HLDD_PECCP: ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K03274, ADP-L-glycero-D-manno-heptose 6-epimerase [EC: 5.1.3.20] (inferred from 100% identity to rah:Rahaq_4341)

MetaCyc: 82% identical to ADP-L-glycero-D-mannoheptose 6-epimerase (Escherichia coli K-12 substr. MG1655)
ADP-glyceromanno-heptose 6-epimerase. [EC: 5.1.3.20]

Predicted SEED Role

"ADP-L-glycero-D-manno-heptose-6-epimerase (EC 5.1.3.20)" in subsystem LOS core oligosaccharide biosynthesis (EC 5.1.3.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.3.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (310 amino acids)

>EX31_RS14395 ADP-glyceromanno-heptose 6-epimerase (Rahnella sp. WP5)
MIIVTGGAGFIGSNIIKSLNDMGYRDILVVDNLKDGTKFVNLVDLDIADYCDKEDFIASI
VAGDDLGDIDAIFHEGACSSTTEWDGKYMMDNNYQYSKDVLHYCLDRSIPFLYASSAATY
GSQSTSFVEERKYEQPLNVYGYSKFLFDQYVREILPHAESQICGFRYFNVYGPREGHKGS
MASVAFHLNNQIKAGENPKLFSGSEGFKRDFIYVGDVAAVNLWFWQNGVSGIFNCGTGRS
ESFQAVADAVVAYHKSGDIEYIPFPEKLKGRYQSFTQADMTALRNAGYDKPFKTVAEGVT
EYMSWLNRTV