Protein Info for EX31_RS14270 in Rahnella sp. WP5

Annotation: glycerol kinase GlpK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 507 transmembrane" amino acids 444 to 460 (17 residues), see Phobius details TIGR01311: glycerol kinase" amino acids 10 to 502 (493 residues), 764.1 bits, see alignment E=2.4e-234 PF00370: FGGY_N" amino acids 11 to 258 (248 residues), 310.2 bits, see alignment E=1.1e-96 PF02782: FGGY_C" amino acids 268 to 456 (189 residues), 152.9 bits, see alignment E=1e-48

Best Hits

Swiss-Prot: 87% identical to GLPK_SALA4: Glycerol kinase (glpK) from Salmonella agona (strain SL483)

KEGG orthology group: K00864, glycerol kinase [EC: 2.7.1.30] (inferred from 100% identity to rah:Rahaq_4316)

MetaCyc: 87% identical to glycerol kinase (Escherichia coli K-12 substr. MG1655)
Glycerol kinase. [EC: 2.7.1.30]

Predicted SEED Role

"Glycerol kinase (EC 2.7.1.30)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or MLST (EC 2.7.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (507 amino acids)

>EX31_RS14270 glycerol kinase GlpK (Rahnella sp. WP5)
MSADTTHEKKYIVALDQGTTSSRAVVLDHDANIVSVSQREFTQIYPKSGWVEHDPMEIWS
SQSSVLVEVLAKADINSDQIAGIGITNQRETTIVWEKETGKPIYNAIVWQCRRTADICEK
LKKDGLEEYIRHNTGLVIDPYFSGTKLKWILDNVEGSRERAKRGELLFGTVDTWLVWKMT
QGRVHVTDYTNASRTMLFNIHDLQWDDRMLEVLDIPREMLPEVRSSSEIYGQTNIGGKGG
TRIPIAGIAGDQQAALYGQLCVQPGMAKNTYGTGCFLLMNTGTEAVASKNGLLTTIACGP
RGEVNYALEGAVFVGGASIQWLRDELKLISDATDSEYFAMKVKDTNGVYVVPAFTGLGAP
YWDPYARGAIFGLSRGANSNHIIRATLESIAYQTRDVLDAMQADSGARLQALRVDGGAVA
NNFLMQFQSDILGTRVERPEVLEVTALGSAFLAGLAVGFWNDLEEVRSKATIEREFRPSI
ETTERNVRYKGWQKAVARVRSWEDHDE