Protein Info for EX31_RS14135 in Rahnella sp. WP5

Annotation: TonB-dependent vitamin B12 receptor BtuB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 625 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR01779: TonB-dependent vitamin B12 receptor" amino acids 3 to 625 (623 residues), 765 bits, see alignment E=3e-234 PF07715: Plug" amino acids 45 to 151 (107 residues), 99 bits, see alignment E=2.3e-32 PF00593: TonB_dep_Rec" amino acids 242 to 599 (358 residues), 158.6 bits, see alignment E=4.8e-50

Best Hits

Swiss-Prot: 74% identical to BTUB_YERPP: Vitamin B12 transporter BtuB (btuB) from Yersinia pestis (strain Pestoides F)

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 100% identity to rah:Rahaq_4287)

MetaCyc: 66% identical to cobalamin outer membrane transporter (Escherichia coli K-12 substr. MG1655)
RXN0-1565; TRANS-RXN0-283

Predicted SEED Role

"Outer membrane vitamin B12 receptor BtuB" in subsystem Coenzyme B12 biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (625 amino acids)

>EX31_RS14135 TonB-dependent vitamin B12 receptor BtuB (Rahnella sp. WP5)
MTIKKHVLLTALSVTAFSGWAQDSTSTNSGDDLVVTANRFPQPVSSVLAPTDIVTRADID
RWQSKSVADVLRRLPGVDIAQNGGMGQQTSLFVRGTNASHTLVLMDGVRLNQAGISGAVD
ISQIPISLVQRIEFIRGPRSAVYGSDAIGGVVNIITTRDKLGTTLTAGIGSNGYQAYDGS
TQQKLGENTVLTVAGNYTYTKGTDVVANLPDNYGDPRQPDRDGFMSKTLWAGLEHQFNDQ
FSGFARAYGFDNRTAYDGTYSYADPAHSDALPDTRQLYSRTYDTGLRFQQGIYSTQISGS
YSNTKDYNYDPKYGPYSASATLDDSDQYNLQWGNTFQVGQGTLSAGADWQKLSTQPGTNY
ITDGYTQRNTGVYATAQQKVSDFTFEGAVRNDDNSQFGSHGTWQSSAAWEFIDGYRLIAS
YGTAFKAPNLGQLYGSFGGNADLKPEESKEWEGGLEGLTGPLNWRLSAYRNDIDQMISYD
NQQFGYYNIDKATIKGVEWTGSFDTGPLRHQVTLEYLDPRNAETNEILLRRAKQQAKYQL
DWTVADFDWSLNYQYLGERYDKNYNTNENVRLGGVSLWDLAVSYPVTSHLTVRGRIANLF
DKDYETVYGYETPGREYNLTGSYTF