Protein Info for EX31_RS13995 in Rahnella sp. WP5

Annotation: 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 110 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details transmembrane" amino acids 36 to 56 (21 residues), see Phobius details amino acids 64 to 84 (21 residues), see Phobius details amino acids 90 to 108 (19 residues), see Phobius details PF00892: EamA" amino acids 35 to 107 (73 residues), 36.4 bits, see alignment E=2.8e-13

Best Hits

Swiss-Prot: 60% identical to ARNE_YERE8: Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE (arnE) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K12962, undecaprenyl phosphate-alpha-L-ara4N flippase subunit ArnE (inferred from 100% identity to rah:Rahaq_2818)

MetaCyc: 51% identical to undecaprenyl-phosphate-alpha-L-Ara4N flippase - ArnE subunit (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-276

Predicted SEED Role

"Polymyxin resistance protein PmrL, sucrose-6 phosphate hydrolase" in subsystem Lipid A-Ara4N pathway ( Polymyxin resistance )

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (110 amino acids)

>EX31_RS13995 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE (Rahnella sp. WP5)
MTGFLLVLVVSVVTCAGQLCQKQAAQVAAHGMARVLRWLGLAILLLGIAMLLWLRVLQLL
PLSLAYPMLSLNFVLITLCARWFFGEVVDGRHWCGVALIMCGIALMSVSL