Protein Info for EX31_RS13815 in Rahnella sp. WP5

Annotation: ribonuclease T

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 238 TIGR01298: ribonuclease T" amino acids 22 to 219 (198 residues), 350.9 bits, see alignment E=1e-109 PF00929: RNase_T" amino acids 31 to 205 (175 residues), 102.3 bits, see alignment E=4.4e-33

Best Hits

Swiss-Prot: 84% identical to RNT_PECAS: Ribonuclease T (rnt) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K03683, ribonuclease T [EC: 3.1.13.-] (inferred from 100% identity to rah:Rahaq_2780)

MetaCyc: 80% identical to ribonuclease T (Escherichia coli K-12 substr. MG1655)
Ribonuclease D. [EC: 3.1.13.5]; 3.1.13.5 [EC: 3.1.13.5]; 3.1.13.- [EC: 3.1.13.5]

Predicted SEED Role

"Ribonuclease T (EC 3.1.13.-)" in subsystem tRNA processing (EC 3.1.13.-)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.5

Use Curated BLAST to search for 3.1.13.- or 3.1.13.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (238 amino acids)

>EX31_RS13815 ribonuclease T (Rahnella sp. WP5)
MRAEFDEDKKLMAENSDINALRGRFRGFYPVVIDVETAGFNAKTDALLEIAAITLKMDED
GWLQSDETVHFHVEPFEGAILVPEALAFNGIDPTNPLRGAVSEYEALHEIFKVIRKGMKE
QNCNRAIIVAHNANFDHSFLMAAAERAGLKRNPFHPFATFDTAALSGLVLGQTVLAKACI
SAGIVFDSSQAHSALYDTERTAELFCELVNRWKRLGGWPLALSDEEVEDETASEENQQ